Tiğli R Seda, Gümüşderelioğlu Menemşe
Chemical Engineering Department, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
J Mater Sci Mater Med. 2009 Mar;20(3):699-709. doi: 10.1007/s10856-008-3624-x. Epub 2008 Nov 6.
In this study, alginate and alginate:chitosan semi interpenetrating polymer network (IPN) scaffolds were prepared by freeze-drying process. Alginate scaffolds were crosslinked with different concentrations of CaCl(2), i.e. 0.5, 1 or 3% (w/v), in 96% (v/v) ethanol solutions for two different periods, i.e. 4 and 24 h, after freeze-drying. Scanning electron microscope (SEM)/ Energy Dispersive Analysis by X-ray (EDAX) analysis and swelling studies indicated that crosslinking of scaffolds with 3% (w/v) CaCl(2) for 24 h was effectively created suitable alginate scaffolds in terms of optimum porosity and mechanical stability. This is why, alginate:chitosan semi IPN scaffolds were prepared at the crosslinking condition mentioned above in 70:30, 60:40 and 50:50% (v/v) alginate:chitosan ratios. Besides the attachment and proliferation abilities of ATDC5 murine chondrogenic cells on alginate, 70:30% (v/v) alginate:chitosan and 50:50% (v/v) alginate:chitosan scaffolds, their cellular responses were assessed for chondrogenic potential. These structural and cellular outcomes demonstrate potential utility of chitosan semi IPNs in alginate scaffolds. Comparative results found in relation to alginate scaffolds, support the necessity for alginate:chitosan scaffolds for improved cartilage tissue engineering.
在本研究中，通过冷冻干燥法制备了藻酸盐以及藻酸盐-壳聚糖半互穿聚合物网络（IPN）支架。藻酸盐支架在冷冻干燥后，于96%（v/v）乙醇溶液中，用不同浓度（即0.5%、1%或3%（w/v））的CaCl₂交联两个不同时长（即4小时和24小时）。扫描电子显微镜（SEM）/X射线能量色散分析（EDAX）以及溶胀研究表明，用3%（w/v）CaCl₂交联24小时能有效制备出在孔隙率和机械稳定性方面合适的藻酸盐支架。因此，在上述交联条件下，按照藻酸盐与壳聚糖体积比为70:30%、60:40%和50:50%（v/v）制备了藻酸盐-壳聚糖半IPN支架。除了评估ATDC5小鼠软骨生成细胞在藻酸盐、70:30%（v/v）藻酸盐-壳聚糖和50:50%（v/v）藻酸盐-壳聚糖支架上的附着和增殖能力外，还对它们的软骨生成潜能细胞反应进行了评估。这些结构和细胞结果证明了壳聚糖半IPN在藻酸盐支架中的潜在应用价值。与藻酸盐支架相关的比较结果，支持了使用藻酸盐-壳聚糖支架改善软骨组织工程的必要性。
