Siqueira José F, Sakamoto Mitsuo, Rosado Alexandre S
Molecular Microbiology Laboratory, Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
Methods Mol Biol. 2010;666:71-85. doi: 10.1007/978-1-60761-820-1_6.
In their natural environments, microorganisms usually live in organized communities. Profiling analysis of microbial communities has recently assumed special relevance as it allows a thorough understanding of the diversity of the microbiota, its behavior over time, and the establishment of patterns associated with health and disease. The application of molecular biology approaches holds the advantage of including culture-difficult and as-yet-uncultivated phylotypes in the profiles, providing a more comprehensive picture of the microbial community. This chapter focuses on two particular techniques: the terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE), both of which have been widely used in environmental studies and have been recently successfully used by the authors in the study of the oral microbial communities associated with conditions of health and disease.
在自然环境中，微生物通常生活在有组织的群落中。微生物群落的分析最近具有特殊意义，因为它能让我们深入了解微生物群的多样性、其随时间的变化情况以及与健康和疾病相关模式的建立。分子生物学方法的应用具有将难以培养和尚未培养的系统发育型纳入分析的优势，从而能更全面地呈现微生物群落的情况。本章重点介绍两种特定技术：末端限制性片段长度多态性分析（T-RFLP）和变性梯度凝胶电泳（DGGE），这两种技术在环境研究中都已得到广泛应用，并且作者最近成功地将它们用于研究与健康和疾病状况相关的口腔微生物群落。
