Liu Nenrong, Chen Gang, Chen Jianxin, Yan Jun, Zhuo Shuangmu, Zheng Liqin, Jiang Xingshan
Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
Scanning. 2010 Nov-Dec;32(6):347-50. doi: 10.1002/sca.20194. Epub 2010 Aug 17.
In this paper, multiphoton microscopy (MPM), based on two-photon excited fluorescence and second harmonic generation signals, was used to image microstructures of human rectal mucosa and submucosa. The morphology and distribution of the main components in mucosa layer, goblet cells, intestinal glands, and a little collagen fibers have been clearly monitored, and the content and distribution of collagen, elastic fibers, and blood vessels in submucosa layer have also been distinctly obtained. The variation of these components is very relevant to the pathology in gastrointestinal system, especially early rectal cancer. Our results indicate that the MPM technique has the potential application in vivo in the clinical diagnosis and monitoring of early rectal cancer.
在本文中，基于双光子激发荧光和二次谐波产生信号的多光子显微镜（MPM）被用于对人直肠黏膜和黏膜下层的微观结构进行成像。黏膜层中主要成分、杯状细胞、肠腺以及少量胶原纤维的形态和分布已被清晰监测，黏膜下层中胶原、弹性纤维和血管的含量及分布也已明确获得。这些成分的变化与胃肠系统的病理状况密切相关，尤其是早期直肠癌。我们的结果表明，MPM技术在早期直肠癌的临床诊断和监测方面具有体内潜在应用价值。
