Jiang Xingshan, Zhuo Shuangmu, Xu Ren'an, Chen Jianxin
Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China.
Scanning. 2012 May-Jun;34(3):170-3. doi: 10.1002/sca.20284. Epub 2011 Sep 19.
Mouse is an important animal model to investigate skin physiological and pathological states. In this article, multiphoton microscopic imaging of in vivo hair mouse skin based on two-photon excited fluorescence and second harmonic generation was examined. Our results show that multiphoton microscopy can clearly display microstructure of stratum corneum, stratum spinosum, and dermis of in vivo mouse skin. The main components of epidermis and dermis such as corneocytes, spinosum cell, collagen fibers, and hair follicles can be distinctly identified in MPM images. Using the optional HRZ 200 fine focusing stage, thickness of different layers can be easily assessed. The results demonstrate that MPM can be regarded as an efficient method for in vivo investigation of skin physiological and pathological states by using hair mouse animal model.
小鼠是研究皮肤生理和病理状态的重要动物模型。在本文中，对基于双光子激发荧光和二次谐波产生的活体有毛小鼠皮肤进行了多光子显微镜成像研究。我们的结果表明，多光子显微镜可以清晰地显示活体小鼠皮肤角质层、棘层和真皮的微观结构。表皮和真皮的主要成分，如角质形成细胞、棘层细胞、胶原纤维和毛囊，在多光子显微镜图像中可以清晰识别。使用可选的HRZ 200精细聚焦平台，可以轻松评估不同层的厚度。结果表明，通过有毛小鼠动物模型，多光子显微镜可被视为一种用于活体研究皮肤生理和病理状态的有效方法。
