[HLA - DQA1基因多态性与乙型肝炎病毒感染结局的关联]

[Association between HLA-DQA1 gene polymorphism and the outcomes of hepatitis B virus infection].

作者信息

Xun Yun-hao, Guo Jian-chun, Shi Wei-zhen, Shi Jun-ping, Liu Chang-ling

机构信息

Hangzhou 6th Hospital, Hangzhou 310014, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Dec;23(6):430-3.

PMID:20718347

Abstract

OBJECTIVE

To investigate the association between HLA-DQA1 gene polymorphism and the outcomes of hepatitis B virus infection in Chinese Han population.

METHODS

A total of 180 consecutive patients with biopsy-proven hepatitis B virus infection (120 patients with chronic hepatitis B and 60 patients with asymptomatic HBV carrier) and 60 subjects who resolved from HBV infection spontaneously were studied. Genotype of human leukocyte antigen(HLA)-DQA1 was detected by polymerase chain reaction sequence specific primer(PCR-SSP).

RESULTS

(1) The frequency of HLA-DQA1 * 0201 allele in chronic hepatitis B group was significant higher than the frequency in resolved from HBV infection spontaneously group (38.3% vs. 5.8%, P < 0.001, A = 10.04, 95% CI: 4.48-22.48). The frequency of HLA-DQA1 * 0102 allele in chronic hepatitis B group was significant lower than the frequency in resolved from HBV infection spontaneously group (9.6% vs. 36.7%, P < 0.001, A = 0.183, 95% CI: 0.10-0.32). (2) The frequency of HLA-DQA1 * 0201 allele in chronic hepatitis B group was significant higher than the frequency in asymptomatic HBV carrier group (38.3% vs. 7.5%, P < 0.01, A = 7.667, 95% CI:3.7-15.87). The frequency of HLA-DQA1 * 0102 allele in chronic hepatitis B group was significant lower than the frequency in asymptomatic HBV carrier group (20% vs. 9.6%, P < 0.01, A = 0.424, 95% CI: 0.23-0.79).

CONCLUSION

HLA-DQA1 gene polymorphism may play an important role in the outcomes of hepatitis B virus infection in-Chinese Han population. The HLA-DQA1 * 0102 allele could keep individuals away from HBV infection, and HLA-DQA1 * 0201 allele could aggravate persistant infection of HBV and hepatic inflammatory.

摘要

目的

探讨中国汉族人群中HLA - DQA1基因多态性与乙型肝炎病毒感染结局之间的关联。

方法

共研究了180例经活检证实的乙型肝炎病毒感染患者（120例慢性乙型肝炎患者和60例无症状HBV携带者）以及60例自发清除HBV感染的受试者。采用聚合酶链反应序列特异性引物（PCR - SSP）检测人类白细胞抗原（HLA）- DQA1的基因型。

结果

（1）慢性乙型肝炎组中HLA - DQA1 * 0201等位基因频率显著高于自发清除HBV感染组（38.3%对5.8%，P < 0.001，A = 10.04，95%可信区间：4.48 - 22.48）。慢性乙型肝炎组中HLA - DQA1 * 0102等位基因频率显著低于自发清除HBV感染组（9.6%对36.7%，P < 0.001，A = 0.183，95%可信区间：0.10 - 0.32）。（2）慢性乙型肝炎组中HLA - DQA1 * 0201等位基因频率显著高于无症状HBV携带者组（38.3%对7.5%，P < 0.01，A = 7.667，95%可信区间：3.7 - 15.87）。慢性乙型肝炎组中HLA - DQA1 * 0102等位基因频率显著低于无症状HBV携带者组（20%对9.6%，P < 0.01，A = 0.424，95%可信区间：0.23 - 0.79）。