[应用实时逆转录聚合酶链反应法检测及分析159例手足口病患者肠道病毒感染情况]
[Detection and analysis of the enterovirus infection within 159 cases of hand-foot-mouth disease by a real-time reverse transcription-PCR assay].
作者信息
Fu Jin, Zhang Yan-ling, Sun Chun-rong, Liu Yan-yan, Cui Xiao-dai, Wang Tian-you
机构信息
Graduate School of Peking Union Medical College, Beiing, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Dec;23(6):488-90.
OBJECTIVE
To reveal the enterovirus infection within children suffering hand-foot-mouth disease (HFMD) in the Capital Institute of Pediatrics from Aprial to August, 2009, for the sake of clinical diagnosis and treatment.
METHODS
Both throat swab and vesicle fluid were taken respectively from 159 children with HFMD. And RNA were extracted from each sample followed with real-time fluorescence quantitative RT-PCR kits with three reagents: universal enterovirus primer, Coxsackievirus A16 (CA16) primer and enterovirus 71 (EV71) primer. Parts of postivive samples were sequenced and analyzed.
RESULTS
(1) EV genes were detected from 152 cases, of which, 102 cases were positive for CA16 and 43 were positive for EV71. (2) CV16:EV71 was 2.37:1. The positive rates of throat swabs and vesicle fluid samples were not statistically significant. (3) The PCR results were same with that of sequence analysis.
CONCLUSION
The hand-foot-mouth disease recently appeared in our hospital was mainly related to the EV71 or CA16 infection. And the percentage of EV71 infections obviously increased compared to that of 2007.
目的
了解2009年4月至8月首都儿科研究所收治的手足口病（HFMD）患儿肠道病毒感染情况，以指导临床诊断与治疗。
方法
收集159例手足口病患儿的咽拭子和疱疹液，分别提取样本RNA，采用通用肠道病毒引物、柯萨奇病毒A16（CA16）引物和肠道病毒71型（EV71）引物三种试剂的实时荧光定量RT-PCR试剂盒进行检测。对部分阳性样本进行测序分析。
结果
（1）152例检测到肠道病毒基因，其中CA16阳性102例，EV71阳性43例。（2）CV16:EV71为2.37:1。咽拭子和疱疹液样本阳性率差异无统计学意义。（3）PCR结果与测序分析结果一致。
结论
近期我院手足口病主要与EV71或CA16感染有关，与2007年相比，EV71感染比例明显上升。
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