Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Langmuir. 2010 Sep 21;26(18):14854-60. doi: 10.1021/la101967u.
We describe herein successful preparations of monodisperse chitosan microcapsules with hollow structures using the SPG membrane emulsification technique. Two preparation procedures were examined in this study. In the first method, monodisperse calcium alginate microspheres were prepared and then coated with unmodified chitosan. Subsequently, tripolyphosphate treatment was conducted to physically cross-link chitosan and solubilize the alginate core at the same time. In the second method, photo-cross-linkable chitosan was coated onto the monodisperse calcium alginate microspheres, followed by UV irradiation to chemically cross-link the chitosan shell and tripolyphosphate treatment to solubilize the core. For both methods, it was determined that the average diameters of the chitosan microcapsules depended on those of the calcium alginate microparticles and that the microcapsules have hollow structures. In addition, the first physical cross-linking method using tripolyphosphate was found to be preferable to obtain the hollow structure, compared with the second method using chemical cross-linking by UV irradiation. This was because of the difference in the resistance to permeation of the solubilized alginate through the chitosan shell layers.
我们在此描述了使用 SPG 膜乳化技术成功制备具有中空结构的单分散壳聚糖微胶囊。本研究考察了两种制备方法。在第一种方法中，制备了单分散的海藻酸钙微球，然后用未改性壳聚糖进行包覆。随后，进行三聚磷酸钠处理以物理交联壳聚糖并同时溶解藻酸盐核。在第二种方法中，将光交联壳聚糖涂覆到单分散的海藻酸钙微球上，然后进行 UV 照射以化学交联壳聚糖壳层，并进行三聚磷酸钠处理以溶解核。对于这两种方法，均确定壳聚糖微胶囊的平均直径取决于海藻酸钙微球的直径，并且微胶囊具有中空结构。此外，与使用 UV 照射进行化学交联的第二种方法相比，使用三聚磷酸钠进行的第一种物理交联方法更有利于获得中空结构。这是因为溶解的藻酸盐通过壳聚糖壳层的渗透阻力不同。
