Department of Pediatric Dentistry, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China.
Center of Modem Analysis, Nanjing University, Nanjing, People's Republic of China.
Drug Des Devel Ther. 2023 Jan 22;17:167-180. doi: 10.2147/DDDT.S390039. eCollection 2023.
To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group. The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites. The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup. The bonded teeth of each group were then cut into the bonded beams. We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis. Data were analyzed with two-way ANOVA and LSD multiple comparison test ( < 0.05).
Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS ( < 0.05) than the other three groups except that there was not a statistical significance ( > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers ( < 0.05), lower expression of nanoleakage ( < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
Altogether, the CONPs had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability.
评价壳聚糖载橄榄苦苷纳米粒子（CONPs）预处理牙本质对树脂-牙本质粘结界面耐久性的影响。
将 80 颗新鲜无龋的第三磨牙随机分为四组（每组 20 颗）：去离子水（DW）组、壳聚糖（CS）组、洗必泰（CHX）组和 CONP 组。DW、CS、CHX 和 CONP 组的牙本质分别用去离子水、1.0mg/LCS 溶液、2%洗必泰溶液和 CONP 混悬液（用 100mg/L 橄榄苦苷制备）预处理，然后用通用粘结剂和树脂复合材料进行粘结。每组粘结牙随机分为即时亚组和老化亚组。将每组粘结牙切成粘结梁，测量其微拉伸粘结强度（μTBS），原子力显微镜观察粘结界面特征，计算界面纳米渗漏分析选定区域内银颗粒的百分比，并通过原位酶谱分析评价粘结界面内源性明胶酶活性。数据采用双因素方差分析和 LSD 多重比较检验（ < 0.05）。
无论在 24 h 时还是热循环后，CONP 组的 μTBS 均优于其他三组（ < 0.05），除了 24 h 时 CONP 组和 CHX 组之间没有统计学意义（ > 0.05）。此外，CONP 组在热循环前后的混合层具有更高的弹性模量（ < 0.05）、更低的纳米渗漏表达（ < 0.05）和更好的基质金属蛋白酶抑制作用，均优于其他三组。
总之，CONPs 具有作为牙本质底漆的潜力，可有效提高牙本质-树脂粘结的耐久性。
