Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, People's Republic of China.
Immunopharmacol Immunotoxicol. 2011 Jun;33(2):323-8. doi: 10.3109/08923973.2010.508497. Epub 2010 Aug 18.
Tilmicosin, a semi-synthetic macrolide antibiotic that is only used in the veterinary clinic, was evaluated for its immunosuppressive activity on the immune responses to ovalbumin (OVA) in mice. Tilmicosin suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro. BALB/c mice were immunized subcutaneously with OVA on day 1 and 4. Beginning on the day of boosting immunization, the mice were administered intraperitoneally with tilmicosin at a single dose of 10, 30, and 90 mg/kg for 10 consecutive days. On day 14, blood samples were collected for measuring specific total-immunoglobulin G (total-IgG), IgG1, IgG2b, and splenocytes were harvested for determining lymphocyte proliferation and interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-4 production. The results demonstrated that tilmicosin could significantly suppress Con A-induced splenocyte proliferation in a dose-dependent manner, decrease LPS-and OVA-induced splenocyte proliferation only at high concentration, produced less IL-2, IL-4, and IFN-γ as compared to the control in the OVA-immunized mice. Moreover, the OVA-specific IgG, IgG1, and IgG2b levels in the OVA-immunized mice were reduced by tilmicosin. These results suggest that tilmicosin could suppress the cellular and humoral immune response in mice.
替米考星，一种仅用于兽医诊所的半合成大环内酯类抗生素，其免疫抑制活性在小鼠卵清蛋白（OVA）免疫反应中进行了评估。替米考星抑制刀豆蛋白 A（Con A）和脂多糖（LPS）刺激的脾细胞体外增殖。BALB/c 小鼠于第 1 天和第 4 天经皮下免疫 OVA。从加强免疫之日起，小鼠连续 10 天每天腹膜内给予替米考星 10、30 和 90mg/kg 一次。在第 14 天，采集血液样本以测量特定总免疫球蛋白 G（总 IgG）、IgG1、IgG2b，并收获脾细胞以确定淋巴细胞增殖和白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）、IL-4 的产生。结果表明，替米考星可显著抑制 Con A 诱导的脾细胞增殖，呈剂量依赖性，仅在高浓度时抑制 LPS 和 OVA 诱导的脾细胞增殖，与对照组相比，OVA 免疫小鼠的 IL-2、IL-4 和 IFN-γ产生较少。此外，OVA 免疫小鼠的 OVA 特异性 IgG、IgG1 和 IgG2b 水平降低。这些结果表明替米考星可抑制小鼠的细胞和体液免疫反应。
