Zhang Yi-Bin, Qin Feng, Sun Hong-Xiang
College of Animal Sciences, Zhejiang University, Hangzhou 310029, P.R. China.
Chem Biodivers. 2006 Sep;3(9):967-74. doi: 10.1002/cbdv.200690105.
The immunosuppressive activity of the ethanol extract of Semen Persicae (EESP) was studied with respect to specific antibody and cellular response to ovalbumin (OVA) in mice. The effects of EESP on mice splenocyte proliferation in vitro were measured. EESP significantly suppressed concanavalin A (ConA)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. Furthermore, the effects of EESP at three dose levels on the humoral and cellular immune responses in the OVA-immunized mice were examined. ICR Mice were immunized subcutaneously with OVA on day 0 and 14. Starting on the day of immunization, the mice were administered intraperitoneally with EESP at a single dose of 0.25, 0.5, and 1.0 mg, and cyclosporin A (CsA, positive drug) at a single dose of 0.1 mg at intervals of 7 days. On day 28, mitogen- and OVA-induced splenocyte proliferation and OVA-specific antibody level in serum were measured. EESP significantly decreased ConA-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice at the dose of 1.0 mg. Meanwhile, the OVA-specific serum IgG, IgG1, and IgG2b antibody levels in the OVA-immunized mice were markedly reduced by EESP in a dose-dependent manner. The results suggest that EESP could suppress the cellular and humoral immune response in mice, and deserve further research to be developed as immunosuppressant.
研究了桃仁乙醇提取物（EESP）对小鼠针对卵清蛋白（OVA）的特异性抗体及细胞反应的免疫抑制活性。测定了EESP对小鼠脾细胞体外增殖的影响。EESP在体外以浓度依赖的方式显著抑制刀豆蛋白A（ConA）和脂多糖（LPS）刺激的脾细胞增殖。此外，还研究了EESP三个剂量水平对OVA免疫小鼠体液免疫和细胞免疫反应的影响。ICR小鼠在第0天和第14天皮下注射OVA进行免疫。从免疫当天开始，小鼠每隔7天腹腔注射一次EESP，单剂量分别为0.25、0.5和1.0mg，以及单剂量为0.1mg的环孢素A（CsA，阳性药物）。在第28天，测定丝裂原和OVA诱导的脾细胞增殖以及血清中OVA特异性抗体水平。EESP在1.0mg剂量时显著降低了OVA免疫小鼠中ConA、LPS和OVA诱导的脾细胞增殖。同时，EESP以剂量依赖的方式显著降低了OVA免疫小鼠血清中OVA特异性IgG、IgG1和IgG2b抗体水平。结果表明，EESP可抑制小鼠的细胞免疫和体液免疫反应，作为免疫抑制剂值得进一步研究开发。
