Wang Minghao, Liu Yang, Liu Shuli, Wang Enhua
Department of Clinical Medicine, China Medical University. Shenyang 110001, China.
Zhongguo Fei Ai Za Zhi. 2009 Apr 20;12(4):306-11. doi: 10.3779/j.issn.1009-3419.2009.04.09.
To explore the correlation between p120-catenin (p120ctn) and small GTPases in human lung cancer, and their effect on the cell-cell adhesion, we examined the expression patterns of p120ctn and Rac1, which is the core member of small GTPases, and their correlation with clinicopathological factors.
S-P immunohistochemistry, Western Blot, and RT-PCR were used to detect the expression patterns of p120ctn and Rac1 in 138 patients with non-small cell lung cancer (NSCLC) and two kinds of homologous lung cancer cell lines. We also used an in vitro model to evaluate their expression, and to determine whether protein expression correlated with the invasive capacity of lung cancer cell lines.
In lung cancer, the levels of protein and mRNA expression of p120ctn were significantly lower than normal lung tissue, and Rac1 was also found to be higher in tumor tissue than in normal lung tissue. A correlation between abnormal p120ctn and overexpression of Rac1 (Correlation coefficient=0.720, P <0.001) was also associated with malignancy of lung cancer, such as poor differentiation (P =0.022), high TNM stage (P =0.010), and lymph node metastasis (P =0.009) in NSCLC patients. Abnormal expression of p120ctn and overexpression of Rac1 was significantly associated with the high metastatic capacity of BE1 cells.
Abnormal p120ctn expression correlates with Rac1 overexpression, which contributes to the malignancy-related of NSCLC.
为了探究人类肺癌中p120连环蛋白（p120ctn）与小GTP酶之间的相关性及其对细胞间黏附的影响，我们检测了p120ctn和小GTP酶的核心成员Rac1的表达模式，以及它们与临床病理因素的相关性。
采用S-P免疫组织化学、蛋白质印迹法和逆转录聚合酶链反应（RT-PCR）检测138例非小细胞肺癌（NSCLC）患者及两种同源肺癌细胞系中p120ctn和Rac1的表达模式。我们还使用体外模型评估它们的表达，并确定蛋白表达是否与肺癌细胞系的侵袭能力相关。
在肺癌中，p120ctn的蛋白质和mRNA表达水平显著低于正常肺组织，且肿瘤组织中的Rac1也高于正常肺组织。p120ctn异常与Rac1过表达之间的相关性（相关系数=0.720，P<0.001）也与肺癌的恶性程度相关，如NSCLC患者的低分化（P=0.022）、高TNM分期（P=0.010）和淋巴结转移（P=0.009）。p120ctn的异常表达和Rac1的过表达与BE1细胞的高转移能力显著相关。
p120ctn表达异常与Rac1过表达相关，这促进了NSCLC的恶性相关进程。
