Dong Xinjun, Liu Yang, Zhang Yong, Dong Qianze, Miao Yuan, Zhang Junyi, Zhang Di, Wang Enhua
Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, China.
Zhongguo Fei Ai Za Zhi. 2008 Aug 20;11(4):529-33. doi: 10.3779/j.issn.1009-3419.2008.04.035.
p120(ctn) plays an important role in cell adhesion and has a significant association with tumorigenesis. To investigate the expression of p120(ctn) (p120 cateinin) isoforms in lung squamous cell carcinomas and adenocarcinomas, and analyze the correlation between p120(ctn) and clinicopathological parameters.
The expression patterns of p120(ctn) in lung cancer tissues and the corresponding normal lung tissues were examined by reverse transcription-polymerase chain reaction (RT-PCR). Immunofluorescence, RT-PCR, and Western blot were used to investigate the expression of p120(ctn) isoforms in lung cancer cell lines BE1 and LH7.
Compared with corresponding normal lung tissues, lung cancer tissues have significantly lower levels of total mRNA, isoform1 and 3 mRNA (P <0.001, P <0.001, P =0.001). Furthermore, reduction of p120(ctn) isoform 1 mRNA is negatively associated-whereas p120(ctn) isoform 3 is positively associated-with lymphatic metastasis (P =0.01, P =0.029). BE1 cells with 94% metastatic frequency has lower levels of p120(ctn) isoforms than LH7 cells with low metastatic potential.
Reductions of p120(ctn) isoform 1 and 3 mRNA is a common phenomenon in lung cancer tissues and may play a role in metastasis progression of human lung cancer.
p120（连环蛋白）在细胞黏附中起重要作用，且与肿瘤发生密切相关。为了研究p120（连环蛋白）异构体在肺鳞状细胞癌和腺癌中的表达情况，并分析p120（连环蛋白）与临床病理参数之间的相关性。
采用逆转录-聚合酶链反应（RT-PCR）检测肺癌组织及相应正常肺组织中p120（连环蛋白）的表达模式。运用免疫荧光、RT-PCR和蛋白质印迹法研究肺癌细胞系BE1和LH7中p120（连环蛋白）异构体的表达情况。
与相应正常肺组织相比，肺癌组织中总mRNA、异构体1和3 mRNA水平显著降低（P<0.001，P<0.001，P = 0.001）。此外，p120（连环蛋白）异构体1 mRNA的降低与淋巴转移呈负相关，而p120（连环蛋白）异构体3与淋巴转移呈正相关（P = 0.01，P = 0.029）。转移频率为94%的BE1细胞中p120（连环蛋白）异构体水平低于转移潜能低的LH7细胞。
p120（连环蛋白）异构体1和3 mRNA水平降低在肺癌组织中是常见现象，可能在人类肺癌转移进展中发挥作用。
