Xu Yuexin, Liu Dongming, Duan Hongmei, Guan Zhongyan, Han Yuchen
Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China; Current address: Department of Pathology, Beijing Men Tougou District Hospital, Beijing 102300, China.
Zhongguo Fei Ai Za Zhi. 2009 May 20;12(5):375-80. doi: 10.3779/j.issn.1009-3419.2009.05.020.
Annexin II is a calcium-dependent phosphnolipid-binding protein that plays a role in many cellular functions. It has been proven that annexin II has been involved in many human cancers. The aim of this study is to examine the expression of annexin II in lung cancer tissues, and analyze the correlations between annexin II and clinicopathological parameters.
Annexin II expression was examined in 110 lung cancer specimens using immunohistochemistry (SP method). Western Blot was used to detect the expression of annexin II in lung cancer tissues and the corresponding normal lung tissues.
Among 110 lung cancer specimens, 67 samples showed positive expression of annexin II (60.9%). It showed membranous staining in lung squamous cell carcinoma. The positive membranous expression and cytoplasmic expression of annexin II in lung adenocarcinoma mainly showed membranous staining. Annexin II expression level was positively correlated with lymphoid node metastasis (P =0.008) and TNM stages (P =0.002). There was expression of annexin II in both lung cancer tissues and the corresponding normal lung tissues, and in lung cancer tissues, the total levels of annexin II proteins were significantly higher than that in the corresponding normal lung tissues (P <0.001).
The expression of annexin II positively may correlated with lung squamous cell carcinoma and adenocarcinoma development.
膜联蛋白II是一种钙依赖性磷脂结合蛋白，在许多细胞功能中发挥作用。已证实膜联蛋白II与多种人类癌症有关。本研究旨在检测膜联蛋白II在肺癌组织中的表达，并分析膜联蛋白II与临床病理参数之间的相关性。
采用免疫组织化学（SP法）检测110例肺癌标本中膜联蛋白II的表达。采用蛋白质免疫印迹法检测肺癌组织及相应正常肺组织中膜联蛋白II的表达。
110例肺癌标本中，67例膜联蛋白II呈阳性表达（60.9%）。在肺鳞状细胞癌中呈膜性染色。肺腺癌中膜联蛋白II的阳性膜性表达和胞质表达主要呈膜性染色。膜联蛋白II表达水平与淋巴结转移（P =0.008）和TNM分期（P =0.002）呈正相关。肺癌组织及相应正常肺组织中均有膜联蛋白II表达，且肺癌组织中膜联蛋白II蛋白总水平显著高于相应正常肺组织（P <0.001）。
膜联蛋白II的阳性表达可能与肺鳞状细胞癌和腺癌的发生发展相关。
