Yang Zhi, Xu Shaofa, Zhang Haiqing, Liu Zhidong, Han Yi
Department of Thoracic Surgery, Beijing Chest Hospital, Beijing 101149, China.
Zhongguo Fei Ai Za Zhi. 2009 May 20;12(5):397-402. doi: 10.3779/j.issn.1009-3419.2009.05.004.
As invasion and metastasis of cancer seriously threaten human's life, it is of more important clinical significance to understand and evaluate the biologic behavior of cancer correctly. Abnormal expression of integrin and ECM proteins play an important role in invasion and metastasis of cancer. The objective of this study is to analyze the expression of the integrin alpha5, beta1 and ECM proteins in patients with NSCLC and its correlation with lymph node (LN) metastasis as well as prognosis, and to evaluate its roles in the metastasis of tumor.
The expression of integrin alpha5, beta1 and collagen type IV, fibronectin, tenascin was determined by immunohistochemistry. The relationship was analysized that between integrin alpha5, beta1 and ECM proteins expression and clinico-pathologic parameters especially LN metastasis and prognosis.
The expression of collagen type IV was negatively correlated with LN metastasis, and positively to prognosis of patients; The expression of integrin alpha5, beta1 was positively correlated with LN metastasis, and integrin alpha5 was negatively to prognosis of patients. The expression of integrin alpha5 was positively correlated with the expression of integrin beta1, and negatively with the expression of collagen type IV. The expression of collagen type IV in stage I disease P-TNM stage was remarkably higher than that in stage III disease and poordifferentiated disease (P <0.001), but integrin alpha5, beta1 was significantly higher than that in stage III disease and poordifferentiated disease.
Increased expression of integrin alpha5, beta1 and decreased expression of ECM significantly correlated with LN metastasis of NSCLC. Integrin alpha5 and collagen type IV are prognosic factors in patients with NSCLC.
由于癌症的侵袭和转移严重威胁人类生命，正确理解和评估癌症的生物学行为具有更重要的临床意义。整合素和细胞外基质（ECM）蛋白的异常表达在癌症的侵袭和转移中起重要作用。本研究的目的是分析非小细胞肺癌（NSCLC）患者中整合素α5、β1和ECM蛋白的表达及其与淋巴结（LN）转移和预后的相关性，并评估其在肿瘤转移中的作用。
采用免疫组织化学法检测整合素α5、β1以及IV型胶原、纤连蛋白、腱生蛋白的表达。分析整合素α5、β1和ECM蛋白表达与临床病理参数尤其是LN转移和预后之间的关系。
IV型胶原的表达与LN转移呈负相关，与患者预后呈正相关；整合素α5、β1的表达与LN转移呈正相关，整合素α5与患者预后呈负相关。整合素α5的表达与整合素β1的表达呈正相关，与IV型胶原的表达呈负相关。I期疾病（P-TNM分期）中IV型胶原的表达明显高于III期疾病和低分化疾病（P<0.001），但整合素α5、β1在III期疾病和低分化疾病中的表达明显更高。
整合素α5、β1表达增加和ECM表达降低与NSCLC的LN转移显著相关。整合素α5和IV型胶原是NSCLC患者的预后因素。
