Yang Shunfang, Su Jianzhong, Cao Jie, Zhang Peiling, Lu Jianying, Xie Wenhui
Department of Nuclear Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.
Zhongguo Fei Ai Za Zhi. 2009 Jul 20;12(7):753-9. doi: 10.3779/j.issn.1009-3419.2009.07.020.
Lung cancer is a common malignancy and is the major determinant of overall cancer mortality worldwidely. Approximately 70% of lung cancer patients will die from metastatic diseases. The aim of this study is to establish a Chinese lung adenocarcinoma cell line with high metastasis potency for exploring the mechanism of occurrence and development in lung cancer.
The cell came from the pericardial effusion of a fifty-year old male patient with lung adenocarcinoma and the cells in primary culture were obtained successfully. Immunodeficient mice tumorigenicity was assayed. The cell growth curve was mappinged. Analysis of chromosome karyotype was tested. Tumor marker was detected by radioimmunoassay. The gene expression was measured by real-time quantitative PCR.
The first passage cells were planted in immunodeficient mice subcutaneously and the tumorigenesis rate was 100% as well as later passages. Under the microscope, the cell showed larger and semi-suspension, semi-adherence. Approximately 0.8*10(6) cancerous cells were injected into left cardiac ventricle or tail vein of immunodeficient mice resulted start to appear lower limb paralysis and spine swelling deformation in the mice after inoculation two-three weeks. The bone metastasis rate was 90% in the tumor bearing mice by bone scintigraphy and pathology and only pulmonary metastasis 10% at the same time. The chromosome karyotype analysis of the cells was sub-triploid. The tumor marker CEA was detected in higher secretion by radioimmunoassay in the cell culture suspension. Quantitative real-time PCR was used to examined and compared SPC-A-1 lung adenocarcinoma, VEGF-C, IL-6, IL-8, genes were overexpressed. The novel cell line was named CPA-Yang1.
Tne novel strain CPA-Yang1 is an parental cell with characteristics of bone metastasis of Chinese lung adenocarcinoma. It has stable traits, highly metastatic ability and a good experimental model for lung cancer research.
肺癌是一种常见的恶性肿瘤，是全球癌症总体死亡率的主要决定因素。约70%的肺癌患者将死于转移性疾病。本研究的目的是建立一种具有高转移潜能的中国肺腺癌细胞系，以探索肺癌发生发展的机制。
细胞来源于一名50岁男性肺腺癌患者的心包积液，成功获得原代培养细胞。检测免疫缺陷小鼠的致瘤性。绘制细胞生长曲线。检测染色体核型分析。通过放射免疫测定法检测肿瘤标志物。通过实时定量PCR测量基因表达。
将第一代传代细胞皮下接种于免疫缺陷小鼠，致瘤率为100%，后续传代也是如此。在显微镜下，细胞呈较大的半悬浮、半贴壁状态。将约0.8×10⁶个癌细胞注入免疫缺陷小鼠的左心室或尾静脉，接种两到三周后小鼠开始出现下肢麻痹和脊柱肿胀变形。骨闪烁显像和病理学检查显示荷瘤小鼠的骨转移率为90%，同时肺转移率仅为10%。细胞的染色体核型分析为亚三倍体。放射免疫测定法检测到细胞培养悬液中肿瘤标志物癌胚抗原（CEA）分泌较高。采用实时定量PCR检测并比较SPC-A-1肺腺癌、血管内皮生长因子C（VEGF-C）、白细胞介素6（IL-6）、白细胞介素8（IL-8）基因均过表达。该新细胞系命名为CPA-Yang1。
新的CPA-Yang1细胞系是具有中国肺腺癌骨转移特征的亲本细胞。它具有稳定的特性、高转移能力，是肺癌研究的良好实验模型。
