[新型中国人肺腺癌细胞系CPA-Yang2在免疫缺陷小鼠中的建立及鉴定。]

[Establishment and Characterization of a Novel Chinese Human Lung Adenocarcinoma Cell Line CPA-Yang2 in Immunodeficient Mice.].

作者信息

Yang Shunfang, Su Jianzhong, Shi Meiping, Zhao Lanxiang, Zhang Peiling, Cao Jie, Lu Jianying, Xie Wenhui

机构信息

Department of Nuclear Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.

出版信息

Zhongguo Fei Ai Za Zhi. 2009 Oct 20;12(10):1055-60. doi: 10.3779/j.issn.1009-3419.2009.10.01.

DOI:10.3779/j.issn.1009-3419.2009.10.01

PMID:20723342

Abstract

BACKGROUND

The recurrence, metastasis and multidrug resistance (MDR) in lung cancer are the tough problems worldwide. This study was to establish a novel chinese lung adenocarcinoma cell line with high metastasis potential for exploring the mechanism of reccurrence, development and MDR in lung cancer.

METHODS

The cell came from the abdominal dropsy of a fifty-six years old female patient with lung adenocarcinoma and the tumor markers CA125, CYFRA21-1, CEA, NSE were detected to be higher secretion by radioimmunoassay in the abdominal dropsy. Tumorigenicity of immunodeficient mice was confirmed in 8th passage. The cell growth curve was mapped. Analysis of chromosome karyotype was tested. The gene expression was measured by real-time quantitative PCR.

RESULTS

The tumorigenesis rate started at 8th passage in 3/10 immunodeficient mice via subcutaneously and the fully tumorigenicity was at 11th passage as well as later passages. Under the microscope, the cell showed oval-shap and adherence. The chromosome karyotype analysis of the cells was sub-triploid. Approximately 110(6) and 1.510(6) cancerous cells were injected into left cardiac ventricle and tail vein of immunodeficient mice respectively. The results showed multiorgan metastasis in the mice after three-four weeks, including mandible, scapula, humerus, vertebral column, femur, rib and brain, liver, adrenal gland, pulmonary in the mice after inoculation. The bone metastasis rate was 100% in the tumor bearing mice by bone scintigraphy and pathology. Quantitative real-time PCR was used to examined and compared with SPC-A-1 lung adenocarcinoma, ESM1, VEGF-C, IL-6, IL-8, AR genes were overexpressed. The novel cell was named CPA-Yang2

CONCLUSIONS

The characteristics of novel strain CPA-Yang2 is a highly metastasis cell line of Chinese lung adenocarcinoma. It has stable traits, highly metastasis ability and maybe is a MDR lung cancerous cell line. Of course, it's a good experimental model for lung cancer research.

摘要

背景

肺癌的复发、转移和多药耐药（MDR）是全球范围内的棘手问题。本研究旨在建立一种具有高转移潜能的新型中国肺腺癌细胞系，以探索肺癌复发、发展和多药耐药的机制。

方法

细胞来源于一名56岁女性肺腺癌患者的腹腔积液，通过放射免疫分析法检测腹腔积液中肿瘤标志物CA125、CYFRA21-1、CEA、NSE分泌较高。在第8代时确认免疫缺陷小鼠的致瘤性。绘制细胞生长曲线。进行染色体核型分析检测。通过实时定量PCR测量基因表达。

结果

3/10免疫缺陷小鼠在第8代经皮下接种时开始出现致瘤率，在第11代及以后各代达到完全致瘤性。在显微镜下，细胞呈椭圆形且贴壁生长。细胞的染色体核型分析为亚三倍体。分别将约1×10(6)和1.5×10(6)个癌细胞注入免疫缺陷小鼠的左心室和尾静脉。结果显示，接种后三四周小鼠出现多器官转移，包括下颌骨、肩胛骨、肱骨、脊柱、股骨、肋骨以及脑、肝、肾上腺、肺。通过骨闪烁显像和病理学检查，荷瘤小鼠的骨转移率为100%。采用定量实时PCR检测并与SPC-A-1肺腺癌比较，ESM1、VEGF-C、IL-6、IL-8、AR基因过表达。该新型细胞命名为CPA-Yang2。