Zhang Peng, Han Yiping, Huang Ling, Li Qiang, Ma Dalie
Department of Respiratory Medicine, Changhai Hospital, Shanghai 200433, China.
Zhongguo Fei Ai Za Zhi. 2009 Sep 20;12(9):995-9. doi: 10.3779/j.issn.1009-3419.2009.09.010.
To detect the expressions of thyroid transcription factor-1 (TTF-1) and p63 in non-small cell lung cancer (NSCLC) and to evaluate their clinical significance.
The expression of TTF-1 and p63 from 404 NSCLC and 28 benign pulmonary disease (BPD) tissue specimens were detected by immunohistochemical EnVision two-step method, together with their clinicopathologic data.
The positive rate of TTF-1 and p63 protein in NSCLC tissues was 51.7% (209/404) and 37.9% (153/404), respectively, while negative in the BPD group. There was overexpression of TTF-1 in female gender and non-smoking history (P<0.001) and asymptomatic patients (P=0.015). It was more frequently in adenocarcinoma (AdC) with sensitivity of 84.1% and specificity of 89.8%, especially in well or moderately differentiated AdC (P<0.001). The positive rate of p63 was closely related with male gender and smoking history (P<0.001). Its sensitivity and specificity to squamous cell carcinoma (SCC) was 95.5% and 98.8%, respectively, which was positively correlated with differentiation of SCC (P=0.008), but negatively with tumor stage (P=0.002). Logistic multivariate analysis showed smoking history and histological type were significantly associated with TTF-1 and p63 expression. 93.1% of those represent TTF-1(+)/p63(-) were AdC, while 98.6% of TTF-1(-)/p63(+) were SCC. p63 expression was negatively correlated with TTF-1 (P<0.001).
TTF-1 and p63 might be effective bio-markers for AdC and SCC in NSCLC. They may be a useful marker panel for the identification and differential of lung cancer.
检测甲状腺转录因子-1（TTF-1）和p63在非小细胞肺癌（NSCLC）中的表达，并评估其临床意义。
采用免疫组织化学EnVision两步法检测404例NSCLC组织标本和28例良性肺疾病（BPD）组织标本中TTF-1和p63的表达，并结合其临床病理资料进行分析。
NSCLC组织中TTF-1和p63蛋白的阳性率分别为51.7%（209/404）和37.9%（153/404），而在BPD组均为阴性。TTF-1在女性、无吸烟史（P<0.001）及无症状患者（P=0.015）中呈过表达。在腺癌（AdC）中更为常见，敏感性为84.1%，特异性为89.8%，尤其在高分化或中分化AdC中（P<0.001）。p63的阳性率与男性及吸烟史密切相关（P<0.001）。其对鳞状细胞癌（SCC）的敏感性和特异性分别为95.5%和98.8%，与SCC的分化程度呈正相关（P=0.008），与肿瘤分期呈负相关（P=0.002）。Logistic多因素分析显示吸烟史和组织学类型与TTF-1和p63的表达显著相关。TTF-1(+)/p63(-)者中93.1%为AdC，而TTF-1(-)/p63(+)者中98.6%为SCC。p63表达与TTF-1呈负相关（P<0.001）。
TTF-1和p63可能是NSCLC中AdC和SCC的有效生物标志物。它们可能是用于肺癌鉴别诊断的有用标志物组合。
