硫酸镁对胎儿脐血浆和人脐静脉内皮细胞基质金属蛋白酶-9活性的影响。
The effect of magnesium sulfate on the activity of matrix metalloproteinase-9 in fetal cord plasma and human umbilical vein endothelial cells.
机构信息
Department of Obstetrics and Gynecology, Madigan Army Medical Center, Tacoma, WA, USA.
出版信息
Am J Obstet Gynecol. 2010 Oct;203(4):371.e1-5. doi: 10.1016/j.ajog.2010.06.012. Epub 2010 Aug 16.
OBJECTIVE
Clinical evidence suggests that magnesium sulfate may reduce the risk of fetal neurologic injury in preterm delivery. Matrix metalloproteinase-9 (MMP-9) levels are elevated in preterm labor patients. There is evidence that MMP-9 may break down the blood-brain barrier in humans, causing cytokine mediated cell injury. Our objective was to determine whether the addition of magnesium sulfate attenuates activity of MMP-9, a complex zinc-dependent enzyme, in fetal cord plasma.
STUDY DESIGN
We collected cord plasma in 6 term, unlabored patients. Using enzyme-linked immunosorbent assay, we measured the activity of MMP-9 with varying concentrations of magnesium sulfate added in vitro. Results were verified using a human umbilical cord vein endothelial cell (HUVEC) line.
RESULTS
Addition of physiologic doses of magnesium sulfate (0.07 mg/mL) resulted in a 25% decrease in active MMP-9 (P = .03). In a HUVEC line, magnesium sulfate resulted in a 32% decrease in MMP-9 activity (P = .00012).
CONCLUSION
The addition of magnesium sulfate attenuated MMP-9 activity in cord plasma and in a HUVEC line.
目的
临床证据表明，硫酸镁可能降低早产儿分娩时胎儿神经损伤的风险。基质金属蛋白酶-9（MMP-9）水平在早产患者中升高。有证据表明，MMP-9 可能在人体内破坏血脑屏障，导致细胞因子介导的细胞损伤。我们的目的是确定硫酸镁的添加是否能减弱胎儿脐血浆中 MMP-9（一种复杂的锌依赖性酶）的活性。
研究设计
我们收集了 6 名足月、未临产患者的脐血浆。通过酶联免疫吸附试验，我们测量了体外添加不同浓度硫酸镁时 MMP-9 的活性。结果通过人脐静脉内皮细胞（HUVEC）系进行验证。
结果
添加生理剂量的硫酸镁（0.07mg/mL）可使活性 MMP-9 降低 25%（P =.03）。在 HUVEC 系中，硫酸镁使 MMP-9 活性降低 32%（P =.00012）。
结论
硫酸镁的添加可减弱脐血浆和 HUVEC 系中 MMP-9 的活性。
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