快速生长分枝杆菌杀菌活性检测用 ATP 生物发光快速检测法的建立。
Development of a rapid ATP bioluminescence assay for biocidal susceptibility testing of rapidly growing mycobacteria.
机构信息
Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0056, USA.
出版信息
J Clin Microbiol. 2010 Oct;48(10):3725-8. doi: 10.1128/JCM.01482-10. Epub 2010 Aug 18.
Abstract
An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides.
摘要
本研究通过优化细胞裂解和检测条件，开发了一种基于三磷酸腺苷（ATP）的分枝杆菌杀生剂敏感性检测法。与传统的琼脂平板法相比，该检测法快速（1.5 h），且通过受试者工作特征（ROC）分析显示出高灵敏度和特异性。测试的物种包括免疫分枝杆菌、溃疡分枝杆菌和脓肿分枝杆菌，它们对戊二醛和异噻唑啉酮基测试杀生剂表现出不同的敏感性。
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