Selvaraju Suresh B, Khan Izhar U H, Yadav Jagjit S
Molecular Toxicology Division, Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH 45267-0056, USA.
Appl Environ Microbiol. 2005 Jan;71(1):542-6. doi: 10.1128/AEM.71.1.542-546.2005.
The microbicidal activity of four different biocides was studied in synthetic metalworking fluid (MWF) against Mycobacterium immunogenum, a suspected causative agent for hypersensitivity pneumonitis, and Pseudomonas fluorescens, a representative for the predominant gram-negative bacterial contaminants of MWF. The results indicated that M. immunogenum is more resistant than P. fluorescens to the tested formaldehyde-releasing biocides (Grotan and Bioban), isothiazolone (Kathon), and phenolic biocide (Preventol). Kathon was effective against mycobacteria at lower concentrations than the other three test biocides in MWF. In general, there was a marked increase in biocidal resistance of both the test organisms when present in MWF matrix compared to saline. Increased resistance of the two test organisms to biocides was observed when they were in a mixed suspension (1:1 ratio). The results indicate the protective effect of the MWF matrix against the action of commonly used biocides on the MWF-colonizing microbial species of occupational health significance, including mycobacteria.
研究了四种不同杀菌剂在合成金属加工液（MWF）中对免疫分枝杆菌（一种疑似超敏性肺炎病原体）和荧光假单胞菌（MWF中主要革兰氏阴性细菌污染物的代表菌）的杀菌活性。结果表明，免疫分枝杆菌比荧光假单胞菌对测试的释放甲醛的杀菌剂（Grotan和Bioban）、异噻唑啉酮（Kathon）和酚类杀菌剂（Preventol）更具抗性。在MWF中，Kathon在比其他三种测试杀菌剂更低的浓度下对分枝杆菌有效。总体而言，与在盐溶液中相比，当存在于MWF基质中时，两种测试菌的杀菌抗性均显著增加。当两种测试菌处于混合悬浮液（1:1比例）中时，观察到它们对杀菌剂的抗性增加。结果表明MWF基质对常用杀菌剂作用于具有职业健康意义的MWF定殖微生物物种（包括分枝杆菌）具有保护作用。
