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通过离心加速薄层色谱法对中性脂质和磷脂进行制备规模的分离。

Preparative scale separation of neutral lipids and phospholipids by centrifugally accelerated thin-layer chromatography.

作者信息

Bergheim S, Malterud K E, Anthonsen T

机构信息

Institute of Pharmacy, University of Oslo, Norway.

出版信息

J Lipid Res. 1991 May;32(5):877-9.

PMID:2072045
Abstract

Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters.

摘要

脂质和磷脂混合物通过离心加速制备规模的薄层色谱法(每次运行300-500毫克脂质混合物)进行分离。分离出的脂质和磷脂通过1H和13C核磁共振光谱进行鉴定,其脂肪酸组成通过其甲酯的气相色谱法和气相色谱-质谱法进行测定。

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