Schmidt E D, Das P K, Van der Gaag R, Tigges A J, Van der Loos C M, Koornneef L
Department of Ophthalmology, Academic Medical Centre, Amsterdam, The Netherlands.
J Pathol. 1991 Jun;164(2):135-43. doi: 10.1002/path.1711640207.
Antigen-presenting cells are of crucial importance for the initiation and regulation of regional immune responses. In a previous study, indirect morphological evidence that morphologically normal human orbital tissues contain HLA-DR-positive macrophages, which may represent antigen-presenting cells, has ben obtained. In the present study, these cells were characterized in detail using double immunoenzyme staining techniques with monoclonal antibodies directed against several well-characterized monocyte/macrophage markers and against HLA-DR gene products. The orbital muscular tissues appear to contain numerous HLA-DR, monocyte/macrophage marker double-stained cells, which are considered to be potential antigen-presenting cells. The cells are widely distributed in the connective tissue of all the orbital muscular tissues studied and consist of several subsets with different phenotypes. Furthermore, site-specific differences were shown between recti muscles and the levator/Müller's muscles with respect to the distribution of HLA-DR and one monocyte/macrophage marker (OKM5). Many of the orbital antigen-presenting cells appear to be of the dendritic type and are considered to be of major importance in regulating local orbital immunity.
抗原呈递细胞对于局部免疫反应的启动和调节至关重要。在先前的一项研究中，已获得间接形态学证据，表明形态正常的人类眼眶组织含有HLA-DR阳性巨噬细胞，这些巨噬细胞可能代表抗原呈递细胞。在本研究中，使用双免疫酶染色技术，用针对几种特征明确的单核细胞/巨噬细胞标志物和HLA-DR基因产物的单克隆抗体对这些细胞进行了详细表征。眼眶肌肉组织似乎含有大量HLA-DR、单核细胞/巨噬细胞标志物双染细胞，这些细胞被认为是潜在的抗原呈递细胞。这些细胞广泛分布在所研究的所有眼眶肌肉组织的结缔组织中，由具有不同表型的几个亚群组成。此外，在直肌与提上睑肌/米勒肌之间，就HLA-DR和一种单核细胞/巨噬细胞标志物（OKM5）的分布而言，显示出部位特异性差异。许多眼眶抗原呈递细胞似乎是树突状类型，被认为在调节局部眼眶免疫中起主要作用。
