Xu Hui-Juan, Zhong Ren, Zhao Yan-Xia, Li Xue-Rong, Lu Yuan, Song Ai-Qin, Pang Xiu-Ying, Yao Ru-Yong, Sun Li-Rong
Department of Pediatric Hematology, Central Laboratory, Qingdao University Medical College, Affiliated Hospital, Qingdao, 266003, Shandong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Aug;18(4):887-92.
This study was to investigate the proliferative inhibition and apoptosis of human leukemia HL-60 cells induced by crocin and their possible mechanisms. The cell viability was tested by cell counting. The morphology of HL-60 cells was observed by fluorescence microscopy. The MTT assay was used to evaluate the inhibitory effect of crocin on the growth of HL-60 cells. Flow cytometry was used to measure the cell cycle. RT-PCR was used to detect bcl-2 and bax expression. The results indicated that the growth of HL-60 cells was inhibited remarkably in the dose and time dependent way. When the crocin concentration was higher than 5 mg/ml, the percentage of apoptotic HL-60 cells was not increased, on the contrary this percentage decreased, the cells manifested necrosis. Flow cytometry profiles revealed that cells were blocked in G₀/G₁ phase, the cell proliferation was inhibited obviously at 5 mg/ml. RT-PCR detection revealed that the expression of bcl-2 was down-regulated strikingly and bax was up-regulated. It is concluded that the crocin can inhibit the proliferation of HL-60 cells effectively, and block cells in G₀/G₁ phase. The mechanisms by which crocin induced apoptosis in HL-60 cells may be related to the inhibition of bcl-2 and activation of bax.
本研究旨在探讨西红花苷对人白血病HL-60细胞增殖的抑制作用及诱导凋亡作用及其可能机制。采用细胞计数法检测细胞活力。通过荧光显微镜观察HL-60细胞形态。采用MTT法评价西红花苷对HL-60细胞生长的抑制作用。采用流式细胞术检测细胞周期。采用RT-PCR检测bcl-2和bax表达。结果表明，西红花苷对HL-60细胞生长具有显著的剂量和时间依赖性抑制作用。当西红花苷浓度高于5mg/ml时，HL-60细胞凋亡率未升高，反而降低，细胞表现为坏死。流式细胞术分析显示细胞阻滞于G₀/G₁期，5mg/ml时细胞增殖明显受到抑制。RT-PCR检测显示bcl-2表达明显下调，bax表达上调。结论：西红花苷能有效抑制HL-60细胞增殖，并将细胞阻滞于G₀/G₁期。西红花苷诱导HL-60细胞凋亡的机制可能与抑制bcl-2和激活bax有关。
