Zhao Pei, Luo Chun-li, Wu Xiao-hou, Hu Hong-bo, Lv Chun-fang, Ji Hui-ying
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China.
Zhongguo Zhong Yao Za Zhi. 2008 Aug;33(15):1869-73.
To investigate the proliferation, apoptosis and mechanisms on T24 cell of transitional cell carcinoma of bladder (TCCB) by crocin.
MTT assay was used to evaluate the proliferation of T24 cells. The changes of cell cycle and cell apoptotic percentage were measured by flow cytometry. T24 cells were inoculated into BALB/c nude mice to establish model of carcinoma of bladder. The mice were randomly divided into control group and experimental group. After treatment with 50 mmol x L(-1) crocin, the inhibited growth of tumor was observed. Electronic microscope was used to observe the morphological changes. The expressions of Bcl-2, Bax, Survivin and Cyclin D1 were detected by immunohistochemistry.
The growth of T24 cells was remarkably inhibited after treatment of crocin. Flow cytometric profiles revealed that crocin led to the increase of the cells in G0/G1 phase, the percentage of cell apoptosis was also increased. Crocin could inhibit the growth of BALB/c xenograft tumor. The morphology changes of cell apoptosis were observed. Bcl-2, Cyclin D1 and survivin expressions determined by immunohistochemical staining were down-regulated after treatment with Bax expression up-regulated.
Crocin exerts both in vitro and in vivo anti-cancer effect on TCCB T24 cell line. The mechanisms may change tumour cell cycle and induce tumour cell apoptosis by down-regulating the expression of Bcl-2, Survivin, Cyclin D1 and up-regulating the expression of Bax.
探讨西红花苷对膀胱移行细胞癌T24细胞增殖、凋亡的影响及其机制。
采用MTT法检测T24细胞的增殖情况；应用流式细胞仪检测细胞周期及细胞凋亡率的变化；将T24细胞接种于BALB/c裸鼠建立膀胱癌模型，随机分为对照组和实验组，给予50 mmol·L⁻¹西红花苷处理后，观察肿瘤生长抑制情况；用电子显微镜观察细胞形态学变化；采用免疫组织化学法检测Bcl-2、Bax、Survivin和Cyclin D1的表达。
西红花苷处理后T24细胞生长明显受抑制。流式细胞仪检测显示，西红花苷使G0/G1期细胞增多，细胞凋亡率升高。西红花苷可抑制BALB/c裸鼠移植瘤生长，观察到细胞凋亡的形态学变化。免疫组织化学染色结果显示，处理后Bcl-2、Cyclin D1和Survivin表达下调，Bax表达上调。
西红花苷对膀胱移行细胞癌T24细胞系具有体内外抗癌作用。其机制可能是通过下调Bcl-2、Survivin、Cyclin D1的表达，上调Bax的表达，从而改变肿瘤细胞周期并诱导肿瘤细胞凋亡。
