Department of Electrical Engineering and Computer Science, University of California, Irvine, Irvine, CA 92697, USA.
Anal Biochem. 2011 Jan 15;408(2):342-4. doi: 10.1016/j.ab.2010.08.014. Epub 2010 Aug 17.
Selective messenger RNA (mRNA) profiling of transfected breast cancer gene expression in a living cell is demonstrated. Atomic force microscope (AFM) probe tips are structurally modified to create a dielectrophoretic force that attracts mRNA molecules within the cell nucleus. The tip end is chemically modified to hybridize only to the target mRNA from a pool of molecules within the nucleus. We successfully combined this scheme with standard assay techniques to develop an assay technology that can be used for early disease detection and basic studies in cell biology.
本文展示了在活细胞中转染乳腺癌基因表达的选择性信使 RNA（mRNA）谱分析。原子力显微镜（AFM）探针尖端经过结构修饰，产生一种电介质力，吸引细胞核内的 mRNA 分子。尖端末端经过化学修饰，仅与细胞核内分子库中的靶 mRNA 杂交。我们成功地将该方案与标准检测技术相结合，开发出一种可用于早期疾病检测和细胞生物学基础研究的检测技术。
