Chen J J, Wu R, Yang P C, Huang J Y, Sher Y P, Han M H, Kao W C, Lee P J, Chiu T F, Chang F, Chu Y W, Wu C W, Peck K
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, 107, ROC.
Genomics. 1998 Aug 1;51(3):313-24. doi: 10.1006/geno.1998.5354.
A high-density cDNA microarray with colorimetry detection system to simultaneously monitor the expression of many genes on nylon membrane is described and characterized. To quantify the expression of genes and to isolate differentially expressed genes, the southern hybridization process on filter membranes was employed. The levels of gene expression were represented by color intensities generated by colorimetric reactions in place of hazardous radioisotopes or costly laser-induced fluorescence detection. The gene expression patterns on nylon membranes were digitized by devices such as an economical flatbed scanner or a digital camera. The quantitative information of gene expression was retrieved by image analysis software. Quantitative comparison of the northern dot-blotting method with the microarray system is described. Applications employing single-color detection as well as dual-color detection to isolate differentially expressed genes among thousands of genes are demonstrated.
描述并表征了一种带有比色检测系统的高密度cDNA微阵列，用于在尼龙膜上同时监测多个基因的表达。为了量化基因表达并分离差异表达基因，采用了滤膜上的Southern杂交过程。基因表达水平由比色反应产生的颜色强度表示，取代了危险的放射性同位素或昂贵的激光诱导荧光检测。尼龙膜上的基因表达模式通过诸如经济实惠的平板扫描仪或数码相机等设备进行数字化处理。通过图像分析软件检索基因表达的定量信息。描述了Northern斑点印迹法与微阵列系统的定量比较。展示了采用单色检测以及双色检测在数千个基因中分离差异表达基因的应用。
