Graduate School of Pharmaceutical Sciences, Keio University, 1-5-14 Shibakoen, Minato, Tokyo 105-0011, Japan.
J Chromatogr A. 2010 Sep 17;1217(38):5978-85. doi: 10.1016/j.chroma.2010.07.067. Epub 2010 Aug 3.
Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0 m(2)/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
聚（N-异丙基丙烯酰胺-共-N-叔丁基丙烯酰胺）[P（IPAAm-co-tBAAm）]刷通过表面引发原子转移自由基聚合（ATRP）在具有大孔的聚（羟甲基丙烯酸酯）[水解的聚（甲基丙烯酸缩水甘油酯-乙二醇二甲基丙烯酸酯）]珠上制备，并应用于热敏色谱的固定相。接枝 PHMA 珠的共聚物刷的最佳用量能够分离具有窄峰和高分辨率的肽和蛋白质。通过氮气吸附法发现珠的比表面积为 43.0 m²/g。接枝有 P（IPAAm-co-tBAAm）共聚物刷的 PHMA 珠改善了热敏色谱的固定相，用于肽和蛋白质的全水相分离。
