College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, People's Republic of China.
J Colloid Interface Sci. 2010 Nov 15;351(2):392-7. doi: 10.1016/j.jcis.2010.07.050. Epub 2010 Jul 25.
A surface plasmon resonance biosensor based on ZnO-Au nanocomposites was developed for the detection of rabbit IgG. The ZnO-Au nanocomposites can bind protein by covalent attachment to construct a probe for target analyte. The probe with unique optical properties and good biocompatibility could enhance the sensitivity of SPR biosensor. Under the optimized conditions, the biosensor based on ZnO-Au nanocomposites exhibits a satisfactory response to rabbit IgG in the concentration range of 0.15-20.00 μg ml(-1). For comparison, the biosensor based on Au film and the biosensor based on Au nanoparticles were also studied for the detection of rabbit IgG. The biosensor based on Au film shows a response to rabbit IgG in the concentration range of 2.50-20.00 μg ml(-1). The biosensor based on Au nanoparticles shows a response in the concentration range of 0.30-20.00 μg ml(-1). The biosensor based on ZnO-Au nanocomposites was therefore found to be the most sensitive of the three types of biosensors. The lowest concentration of rabbit IgG that can be determined by the proposed biosensor is about 16-fold lower than that of the biosensor based on Au film alone.
基于 ZnO-Au 纳米复合材料的表面等离子体共振生物传感器被开发用于检测兔 IgG。ZnO-Au 纳米复合材料可以通过共价附着结合蛋白质来构建用于目标分析物的探针。具有独特光学性质和良好生物相容性的探针可以提高 SPR 生物传感器的灵敏度。在优化条件下，基于 ZnO-Au 纳米复合材料的生物传感器对浓度范围为 0.15-20.00 μg ml(-1)的兔 IgG 表现出令人满意的响应。相比之下，还研究了基于 Au 膜和基于 Au 纳米粒子的生物传感器用于检测兔 IgG。基于 Au 膜的生物传感器在浓度范围为 2.50-20.00 μg ml(-1)内对兔 IgG 有响应。基于 Au 纳米粒子的生物传感器在浓度范围为 0.30-20.00 μg ml(-1)内有响应。因此，发现基于 ZnO-Au 纳米复合材料的生物传感器是三种生物传感器中最灵敏的。通过所提出的生物传感器可以检测到的最低浓度的兔 IgG 大约比单独基于 Au 膜的生物传感器低 16 倍。
