State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Beijing East Road, 71, Nanjing 210008, PR China.
J Hazard Mater. 2010 Nov 15;183(1-3):718-23. doi: 10.1016/j.jhazmat.2010.07.085. Epub 2010 Jul 30.
The goal of this study was to isolate PAHs degraders that can utilize PAHs associated with soil particulates and investigate the biodegradation of PAHs on agar plate, in liquid culture and soil. Two Mycobacterium strains (NJS-1 and NJS-P) were isolated from PAHs-contaminated farmland soil using enrichment based on soil slurry. The isolates could degrade five test PAHs including pyrene, phenanthrene, fluoranthene, anthracene and benzo[a]pyrene on plate, but showed different effects in liquid culture, especially for fluoranthene. Isolate NJS-1 was capable of utilizing benzo[a]pyrene as a sole carbon and energy source, and an enhanced degradation was observed when pyrene was supplied as cometabolic substrate. Reintroduction of the isolates into sterile contaminated soil resulted in a significant removal of aged pyrene and fluoranthene (over 40%) in 2-months incubation. In pyrene-spiked soil, the degradation of pyrene and fluoranthene increased to 90% and 50%, respectively. Comparing PAHs degradation on plate, in liquid culture and soil, we can conclude that there was corresponding degradation in different test systems. In addition, the degradation of aged PAHs in soil suggested the potential application of two isolates in further bioremediation.
本研究的目的是分离能够利用与土壤颗粒相关的多环芳烃的降解菌，并研究多环芳烃在琼脂平板、液体培养和土壤中的生物降解情况。从多环芳烃污染农田土壤中采用基于土壤泥浆的富集法分离到两株分枝杆菌（NJS-1 和 NJS-P）。这些分离物能够在平板上降解五种测试多环芳烃，包括芘、菲、荧蒽、蒽和苯并[a]芘，但在液体培养中表现出不同的效果，特别是对于荧蒽。分离物 NJS-1 能够利用苯并[a]芘作为唯一的碳源和能源，并且当提供芘作为共代谢底物时，观察到增强的降解。将分离物重新引入无菌污染土壤中，在 2 个月的孵育期内导致老化的芘和荧蒽（超过 40%）的显著去除。在添加芘的土壤中，芘和荧蒽的降解分别增加到 90%和 50%。比较平板、液体培养和土壤中的多环芳烃降解情况，可以得出在不同的测试系统中存在相应的降解。此外，土壤中老化多环芳烃的降解表明这两个分离物在进一步生物修复中的潜在应用。
