Hu Feng-chai, Li Xin-yu, Su Zhen-cheng, Wang Xiu-juan, Zhang Hui-wen, Sun Jun-de
College of Land and Environment, Shenyang Agricultural University, Shenyang 110161, China.
Ying Yong Sheng Tai Xue Bao. 2011 Jul;22(7):1857-62.
Three pyrene-degrading bacterial strains named D44, D82S and D82Q were isolated from PAHs-contaminated soil in Shenfu Irrigation Area of Shenyang, Northeast China. The strains were identified as Gordonia sp., based on the morphological observation, physiological and biochemical identification, and phylogenetical analysis of 16S rDNA sequences. For all the three stains, their optimal pH was 7, and their growth was obviously inhibited when the pH was lower than 5 or higher than 9. The three strains were capable of utilizing pyrene, benzo[a] pyrene, anthracene, naphthalene, phenanthrene, and fluoranthene as the sole source of carbon and energy. After seven days incubation, the three strains could degrade more than 65% of pyrene with an initial concentration 100 mg x L(-1), and the D44, D82S, and D82Q could degrade 79.6%, 91.3%, and 62.8% of benzo[a] pyrene with an initial concentration 50 mg x L(-1), respectively. PCR amplification indicated that the strains D82Q and D82S possessed alkane monooxygenase gene alkB.
从中国东北沈阳沈抚灌区多环芳烃污染土壤中分离出三株芘降解细菌菌株，分别命名为D44、D82S和D82Q。通过形态学观察、生理生化鉴定以及16S rDNA序列的系统发育分析，将这些菌株鉴定为戈登氏菌属。对于所有这三株菌株，它们的最适pH为7，当pH低于5或高于9时，其生长明显受到抑制。这三株菌株能够利用芘、苯并[a]芘、蒽、萘、菲和荧蒽作为唯一碳源和能源。经过7天培养，当初始浓度为100 mg·L(-1)时，这三株菌株对芘的降解率均超过65%；当初始浓度为50 mg·L(-1)时，D44、D82S和D82Q对苯并[a]芘的降解率分别为79.6%、91.3%和62.8%。PCR扩增表明，菌株D82Q和D82S拥有烷烃单加氧酶基因alkB。
