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胰岛素样生长因子-1、转化生长因子-α加转化生长因子-β1和碱性成纤维细胞生长因子对促卵泡素刺激的水牛腔前卵泡体外存活、生长及凋亡的影响

Effects of IGF-1, TGF-alpha plus TGF-beta1 and bFGF on in vitro survival, growth and apoptosis in FSH-stimulated buffalo (Bubalis bubalus) preantral follicles.

作者信息

Sharma G Taru, Dubey Pawan K, Kumar G Sai

机构信息

Reproductive Physiology and ETT Laboratory, Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, India.

出版信息

Growth Horm IGF Res. 2010 Aug;20(4):319-25. doi: 10.1016/j.ghir.2010.05.001.

DOI:10.1016/j.ghir.2010.05.001
PMID:20726112
Abstract

OBJECTIVE

Investigate the effect of various growth factors viz. IGF-I, TGF-alpha + TGF-beta1 and bFGF either alone or in combination, with FSH on in vitro growth, survival, antrum formation, steroidogenesis and apoptosis of buffalo preantral follicles (PFs).

METHODS

Buffalo ovaries were collected from abattoir; PFs were isolated and divided into five treatment groups. TCM-199 supplemented with 10% FBS, 1% ITS+EGF+FSH control (group a), control+IGF-I (group b), control + TGF-alpha + TGF-beta1 (group c), control + IGF-I + TGF-alpha + TGF-beta1 (group d) and control+bFGF (group e). Progesterone (P4) and 17beta-estradiol (E2) concentrations were evaluated by RIA and apoptosis by TUNEL assay.

RESULTS

TGF-alpha + TGF-beta1 inhibited follicular survival and induced oocyte apoptosis, while IGF-I + TGF-alpha + TGF-beta1 suppressed this inhibitory action. IGF-I significantly (P < 0.05) enhanced the follicle survival, growth and induced antrum formation. FGF had greater effects on both survival and growth rate of oocytes than other treatment groups. Progesterone and estradiol accumulation was significantly (P < 0.05) greater in presence of FGF and IGF-I than TGF-alpha + TGF-beta1.

CONCLUSION

Survival, growth, antrum formation and steroidogenesis are stimulated by IGF-I and bFGF, whereas TGF-alpha + TGF-beta1 inhibited growth and survival of PFs which led to induced oocyte apoptosis in buffalo PFs.

摘要

目的

研究各种生长因子，即胰岛素样生长因子-I（IGF-I）、转化生长因子-α（TGF-α）+转化生长因子-β1（TGF-β1）和碱性成纤维细胞生长因子（bFGF）单独或联合促卵泡素（FSH）对水牛腔前卵泡（PFs）体外生长、存活、腔形成、类固醇生成和凋亡的影响。

方法

从屠宰场收集水牛卵巢；分离PFs并分为五个处理组。添加10%胎牛血清（FBS）、1%胰岛素-转铁蛋白-硒（ITS）+表皮生长因子（EGF）+FSH的TCM-199作为对照（a组），对照+IGF-I（b组），对照+TGF-α+TGF-β1（c组），对照+IGF-I+TGF-α+TGF-β1（d组），对照+bFGF（e组）。通过放射免疫分析法（RIA）评估孕酮（P4）和17β-雌二醇（E2）浓度，通过末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测凋亡。

结果

TGF-α+TGF-β1抑制卵泡存活并诱导卵母细胞凋亡，而IGF-I+TGF-α+TGF-β1抑制了这种抑制作用。IGF-I显著（P<0.05）提高了卵泡存活率、生长率并诱导了腔形成。与其他处理组相比，FGF对卵母细胞的存活和生长率影响更大。与TGF-α+TGF-β1相比，在FGF和IGF-I存在时，孕酮和雌二醇的积累显著（P<0.05）更多。

结论

IGF-I和bFGF刺激了水牛PFs的存活、生长、腔形成和类固醇生成，而TGF-α+TGF-β1抑制了PFs的生长和存活，导致卵母细胞凋亡。

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