El Serougy Iman M, Shahin Amira A, Soliman Doaa A, Akhnoukh Amany F, Mousa Somaia M
Department of Rheumatology & Rehabilitation, Kasr Al-Aini School of Medicine, Cairo University, Cairo, Egypt.
Egypt J Immunol. 2009;16(1):1-8.
The pathogenesis of scleroderma encompasses vascular, immunological, and fibrotic processes, which contribute to clinical manifestations. We investigated the prevalence of anti-annexin V IgG and IgM antibodies in sera of scleroderma patients and their relation to the presence of other antibodies and development of disease morbidity. Sera of 40 scleroderma patients and 15 healthy controls were examined for IgG and IgM anti-annexin V antibodies by ELISA and anticentromere antibodies by indirect immunofluorescence. Serum level of anti-annexin V IgG antibodies in scleroderma patients was significantly higher than that of the control (P < 0.001) and correlated significantly with the presence of digital ischemia (P = 0.023) and pulmonary fibrosis (P = 0.02). IgM isotype was comparable between patients and controls (P = 0.317). Anticentromere antibodies are more prevalent in the limited cutaneous subtype (P = 0.017). In conclusion, measurement of serum anti-annexin V IgG antibodies in scleroderma patients may be important for early diagnosis of vascular and pulmonary complications.
硬皮病的发病机制包括血管、免疫和纤维化过程，这些过程导致了临床表现。我们研究了硬皮病患者血清中抗膜联蛋白V IgG和IgM抗体的患病率及其与其他抗体的存在和疾病发病率发展的关系。通过酶联免疫吸附测定（ELISA）检测40例硬皮病患者和15例健康对照血清中的IgG和IgM抗膜联蛋白V抗体，并通过间接免疫荧光检测抗着丝点抗体。硬皮病患者血清抗膜联蛋白V IgG抗体水平显著高于对照组（P < 0.001），且与指端缺血（P = 0.023）和肺纤维化（P = 0.02）的存在显著相关。患者和对照组之间的IgM同种型无差异（P = 0.317）。抗着丝点抗体在局限性皮肤亚型中更为常见（P = 0.017）。总之，检测硬皮病患者血清抗膜联蛋白V IgG抗体可能对血管和肺部并发症的早期诊断具有重要意义。
