Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Casilla de Correo 16, Sucursal 4, (1900) La Plata, Argentina.
ACS Appl Mater Interfaces. 2010 Sep;2(9):2530-9. doi: 10.1021/am100313z.
This paper describes bacterial organization on nano/micropatterned surfaces with different chemical properties, which show different interactions with the biological systems (inert, biocompatible, and bactericide). These surfaces were prepared by molding techniques and exposed to Pseudomonas fluorescens (P. fluorescens) cultures. Results from atomic force microscopy and optical imaging demonstrate that the structure of P. fluorescens aggregates is strongly dependent on the surface topography while there is no clear linking with the physical-chemical surface properties (charge and contact angle) of the substrate immersed in abiotic culture media. We observe that regardless of the material when the surface pattern matches the bacterial size, bacterial assemblages involved in surface colonization are disorganized. The fact there is not a relationship between surface chemistry and bacterial organization can be explained by the coverage of the surfaces by adsorbed organic species coming from the culture medium. Viability assays indicate that copper behaves as a toxic substrate despite the presence of adsorbed molecules. The combination of surface traps and biocidal activity could act synergistically as a suitable strategy to limit bacterial spreading on implant materials.
本文描述了具有不同化学性质的纳米/微图案表面上的细菌组织，这些表面与生物系统（惰性、生物相容和杀菌）表现出不同的相互作用。这些表面是通过成型技术制备的，并暴露于荧光假单胞菌（P. fluorescens）培养物中。原子力显微镜和光学成像的结果表明，P. fluorescens 聚集体的结构强烈依赖于表面形貌，而与浸入非生物培养基中的基底的物理化学表面性质（电荷和接触角）没有明显的联系。我们观察到，无论材料如何，当表面图案与细菌大小相匹配时，参与表面定殖的细菌聚集物是无序的。表面化学与细菌组织之间没有关系的事实可以用来自培养基的吸附有机物质覆盖表面来解释。生存能力测定表明，尽管存在吸附分子，但铜仍表现出毒性底物的特性。表面陷阱和杀菌活性的结合可以协同作用，作为一种限制细菌在植入材料上扩散的合适策略。
