Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, USA.
Langmuir. 2010 Sep 21;26(18):14427-9. doi: 10.1021/la1030334.
A biotin-tetramethylrhodamine (biotin-TMR) quencher-ligand interacts with a (phenylene-ethynylene) based helical conjugated polyelectrolyte (poly-1) via intercalation of the TMR unit into the helix. The interaction is signaled by efficient fluorescence resonance energy transfer (FRET) from the polymer to the TMR chromophore. Avidin addition to the poly-1/biotin-TMR intercalation complex does not interrupt FRET, instead resulting in the formation of avidin-biotin "cross-links". Mixing of biotin-TMR with avidin prior to addition of the polymer efficiently disrupts the FRET signal, giving rise to a sensor with a detection limit of 100 pM for avidin. Study of the FRET response as a function of biotin-TMR and avidin concentration affords insight into the interaction of the protein with the poly-1/biotin-TMR intercalation complex.
生物素-四甲基罗丹明（biotin-TMR）猝灭剂配体通过 TMR 单元嵌入螺旋体与基于（苯乙炔）的螺旋共轭聚合物（poly-1）相互作用。该相互作用通过聚合物到 TMR 发色团的有效荧光共振能量转移（FRET）发出信号。亲和素添加到 poly-1/biotin-TMR 嵌入复合物中不会中断 FRET，反而会导致形成亲和素-生物素“交联”。在添加聚合物之前将 biotin-TMR 与亲和素混合可有效破坏 FRET 信号，从而产生检测限为 100 pM 的亲和素传感器。作为 biotin-TMR 和亲和素浓度的函数研究 FRET 响应，深入了解了该蛋白质与 poly-1/biotin-TMR 嵌入复合物的相互作用。
