Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
OMICS. 2010 Aug;14(4):369-84. doi: 10.1089/omi.2010.0053.
SOX2 is an HMG box containing transcription factor that has been implicated in various types of cancer, but its role in colorectal cancers (CRC) has not been studied. Here we show that SOX2 is overexpressed in CRC tissues compared with normal adjacent tissues using immunohistochemical staining and RT-PCR. We also observed an increased SOX2 expression in nucleus of colorectal cancer tissues (46%, 14/30 cases vs. 7%, 2/30 adjacent tissues). Furthermore, knockdown of SOX2 in SW620 colorectal cancer cells decreased their growth rates in vitro cell line, and in vivo in xenograft models. ChIP-Seq analysis of SOX2 revealed a consensus sequence of wwTGywTT. An integrated expression profiling and ChIP-seq analysis show that SOX2 is involved in the BMP signaling pathway, steroid metabolic process, histone modifications, and many receptor-mediated signaling pathways such as IGF1R and ITPR2 (Inositol 1,4,5-triphosphate receptor, type 2).
SOX2 是一种含有 HMG 盒的转录因子，已被牵涉到多种类型的癌症中，但它在结直肠癌（CRC）中的作用尚未得到研究。在这里，我们通过免疫组织化学染色和 RT-PCR 显示，SOX2 在 CRC 组织中的表达高于正常相邻组织。我们还观察到在结直肠癌细胞核中 SOX2 的表达增加（46％，14/30 例与 7％，2/30 例相邻组织）。此外，在 SW620 结直肠癌细胞中敲低 SOX2 会降低其体外细胞系和异种移植模型中的生长速度。SOX2 的 ChIP-Seq 分析显示出 wwTGywTT 的共识序列。综合表达谱和 ChIP-seq 分析表明，SOX2 参与了 BMP 信号通路、类固醇代谢过程、组蛋白修饰以及许多受体介导的信号通路，如 IGF1R 和 ITPR2（三磷酸肌醇受体，2 型）。
