Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
Res Vet Sci. 2011 Jun;90(3):404-11. doi: 10.1016/j.rvsc.2010.07.007. Epub 2010 Aug 21.
This study aims to investigate the effects of follicle stimulating hormone (FSH) and fibroblast growth factor-2 (FGF-2) on the survival and growth of caprine preantral follicles. Ovarian tissues were cultured for 1, 7, 14, 21 or 28 days in medium supplemented with FSH (FSH-2d or FSH-7d, i.e., with replacement of the culture medium every 2 or 7 days, respectively) or FSH+FGF-2 (replacement of the medium every 2 days). Non-cultured (control) and cultured ovarian fragments were processed for histological and ultrastructural analysis. After 28 days of culture, the media supplemented with FSH-2d was the most effective in maintaining the percentage of normal follicles and in promoting follicular growth. Furthermore, both treatments with FSH increased the percentage of the primary follicles. However, ultrastructural studies did not confirm follicular integrity from 14 days of culture onward. In conclusion, culturing tissue for up to 7 days in medium containing FSH alone or combined with FGF-2 maintains caprine preantral follicle integrity and promotes their growth in vitro.
本研究旨在探讨卵泡刺激素（FSH）和纤维母细胞生长因子-2（FGF-2）对山羊腔前卵泡存活和生长的影响。卵巢组织在含有 FSH 的培养基中培养 1、7、14、21 或 28 天，培养基中分别补充 FSH（FSH-2d 或 FSH-7d，即分别每 2 天或 7 天更换培养基）或 FSH+FGF-2（每 2 天更换培养基）。非培养（对照）和培养的卵巢组织片段进行组织学和超微结构分析。培养 28 天后，FSH-2d 补充培养基最有效地维持正常卵泡的百分比，并促进卵泡生长。此外，两种 FSH 处理均增加了初级卵泡的百分比。然而，超微结构研究并未证实从培养的第 14 天开始卵泡的完整性。总之，在含有 FSH 的培养基中培养组织长达 7 天，单独或与 FGF-2 联合使用，可维持山羊腔前卵泡的完整性，并促进其体外生长。
