Matos M H T, van den Hurk R, Lima-Verde I B, Luque M C A, Santos K D B, Martins F S, Báo S N, Lucci C M, Figueiredo J R
Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Brazil.
Cells Tissues Organs. 2007;186(2):112-20. doi: 10.1159/000103016. Epub 2007 May 23.
The aims of the present study were to evaluate the effects of fibroblast growth factor-2 (FGF-2) on survival, activation and growth of caprine early-staged (preantral) follicles using histological and ultrastructural studies. Fragments of caprine ovarian cortex were cultured for 1 or 5 days in an enriched minimum essential medium, supplemented or not with different concentrations of FGF-2 (10, 50 or 100 ng/ml). Fragments from non-cultured ovarian tissue (control) and from tissues cultured for 1 or 5 days in a specific medium were processed for transmission electron microscopy (TEM) or classical histology to evaluate the morphological quality of caprine preantral follicles and to calculate the percentages of normal follicles. Additionally, effects of FGF-2 on oocyte and follicle diameter of cultured preantral follicles were investigated. Our results showed that, although the percentages of histologically normal follicles were lower in cultured than in non-cultured ovarian tissue fragments, there were no differences in this regard among treatments, neither on day 1 nor on day 5 of culture. After 1 and 5 days of culture, a significantly higher percentage of growing follicles was observed in the medium supplemented with 50 ng/ml of FGF-2. This FGF-2 treatment furthermore resulted in an increase in diameter of both oocytes and follicles that were cultured for 5 days. TEM showed that the ultrastructural integrity of caprine preantral follicles was maintained during their 5-day culture in the presence of 50 ng/ml FGF-2. In conclusion, this study demonstrated that at a concentration of 50 ng/ml FGF-2 not only maintains the morphological integrity of caprine preantral follicles cultured for 5 days, but also stimulates the activation of primordial follicles and the growth of activated follicles.
本研究的目的是通过组织学和超微结构研究，评估成纤维细胞生长因子-2（FGF-2）对山羊早期（窦前）卵泡存活、激活和生长的影响。将山羊卵巢皮质碎片在富集的最低限度基本培养基中培养1天或5天，培养基中添加或不添加不同浓度的FGF-2（10、50或100 ng/ml）。对未培养的卵巢组织（对照）以及在特定培养基中培养1天或5天的组织碎片进行透射电子显微镜（TEM）或经典组织学处理，以评估山羊窦前卵泡的形态质量，并计算正常卵泡的百分比。此外，还研究了FGF-2对培养的窦前卵泡卵母细胞和卵泡直径的影响。我们的结果表明，尽管培养的卵泡在组织学上正常的百分比低于未培养的卵巢组织碎片，但在培养第1天和第5天，各处理组之间在这方面没有差异。培养1天和5天后，在添加50 ng/ml FGF-2的培养基中观察到生长卵泡的百分比显著更高。这种FGF-2处理还导致培养5天的卵母细胞和卵泡直径增加。TEM显示，在50 ng/ml FGF-2存在的情况下，山羊窦前卵泡在其5天培养过程中维持了超微结构的完整性。总之，本研究表明，浓度为50 ng/ml的FGF-2不仅能维持培养5天的山羊窦前卵泡的形态完整性，还能刺激原始卵泡的激活和激活卵泡的生长。
