Institute of Microelectronics NCSR Demokritos, Terma Patriarchou Grigoriou, Aghia Paraskevi, 15310 Athens, Greece.
Biosens Bioelectron. 2010 Dec 15;26(4):1588-92. doi: 10.1016/j.bios.2010.07.119. Epub 2010 Aug 4.
The detection of DNA hybridization using capacitive readout and a biosensor array of ultrathin Si membranes is presented. The biosensor exploits the ability of the ultrathin membranes to deflect upon surface stress variations caused by biological interactions. Probe DNA molecules are immobilized on the membrane surface and the surface stress variations during hybridization with their complementary strands force the membrane to deflect and effectively change the capacitance between the flexible membrane and the fixed substrate. The sensor array comprises 256 such sensing sites thus allowing the concurrent sensing of multiple DNA mutations. The biosensor and its performance for the detection of complementary DNA strands are demonstrated using beta-thalassemia oligonucleotides. The experimental results show that the presented sensors are able to detect DNA hybridization and to discriminate single nucleotide mismatches.
本文提出了一种使用电容读出和超薄 Si 膜生物传感器阵列检测 DNA 杂交的方法。该生物传感器利用超薄膜在生物相互作用引起的表面应力变化时发生弯曲的能力。探针 DNA 分子被固定在膜表面上，在与互补链杂交时，表面应力变化会迫使膜弯曲，从而有效地改变柔性膜和固定基底之间的电容。传感器阵列包含 256 个这样的传感位点，从而可以同时检测多个 DNA 突变。本文使用β-地中海贫血寡核苷酸演示了该生物传感器及其用于检测互补 DNA 链的性能。实验结果表明，所提出的传感器能够检测 DNA 杂交并区分单个核苷酸错配。
