Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-Dong, Mapo-Gu, Seoul 121-742, Republic of Korea.
Biosens Bioelectron. 2010 Dec 15;26(4):1486-92. doi: 10.1016/j.bios.2010.07.089. Epub 2010 Jul 30.
In vitro assays have generally been carried out for cytological diagnosis and for evaluation of the cytotoxic effect of chemotherapeutic agents as an alternative to animal experiments. In this study, a method for fabrication and application of a gold nanoflower array on an ITO substrate for evaluation of the effect of chemotherapeutic agents on cancer cell behavior by the surface-enhanced Raman scattering (SERS) analysis, as well as the electrochemical detection was described. Due to the increased sensitivity provided by gold nanoflower substrates, the effect of chemotherapeutic agents at low concentration level was successfully detected based on SERS technique. This substrate was found to give enhanced Raman spectra with high surface plasmon field in the near infrared (NIR) spectral range, which minimize fluorescence interference and photo-toxicity. Cyclic voltammetry (CV) was further performed for confirmation of results obtained by SERS assay and showed increased intensity of current peaks for various concentrations at low levels. The developed Au nanoflowers modified ITO substrates developed in this study could be used as a simultaneous SERS and CV substrate to determine the effects of chemotherapeutic agents on cancer cells.
体外分析通常用于细胞学诊断和评估化疗药物的细胞毒性作用，作为动物实验的替代方法。在这项研究中，描述了一种在 ITO 基底上制造和应用金纳米花阵列的方法，用于通过表面增强拉曼散射（SERS）分析评估化疗药物对癌细胞行为的影响，以及电化学检测。由于金纳米花基底提供了更高的灵敏度，因此成功地基于 SERS 技术检测到低浓度水平的化疗药物的作用。该基底在近红外（NIR）光谱范围内产生具有高表面等离激元场的增强拉曼光谱，最大限度地减少荧光干扰和光毒性。进一步进行循环伏安法（CV）以确认 SERS 分析获得的结果，并显示在低浓度下各种浓度的电流峰强度增加。本研究中开发的 Au 纳米花修饰的 ITO 基底可用作同时进行 SERS 和 CV 基底，以确定化疗药物对癌细胞的影响。
