神经元分化过程中 NT2 多潜能人胚胎癌细胞基因的可变剪接。
Alternative splicing of genes during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells.
机构信息
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
FEBS Lett. 2010 Sep 24;584(18):4041-7. doi: 10.1016/j.febslet.2010.08.024. Epub 2010 Aug 20.
We analyzed the mRNA diversity of genes after inducing neuronal differentiation in human NT2 teratocarcinoma cells using all-trans retinoic acid (RA). DNA microarray analyses of cells treated with RA identified 358 RA-responsive genes. mRNA diversity analysis revealed that 274 genes produced multiple protein-coding transcripts by alternative splicing. Among these 274 genes, we chose 26 genes that showed AS in their C-terminus and 12 transcription factor genes for further analysis. By using transcript-specific primers, we performed quantitative real-time PCR analysis to examine the expression profiles of all the protein-coding transcripts. Consequently, we identified genes which showed different RA-induced changes in the expression of their protein-coding transcripts.
我们使用全反式视黄酸（RA）诱导人 NT2 畸胎瘤细胞向神经元分化，分析了神经元分化后基因的 mRNA 多样性。用 RA 处理的细胞的 DNA 微阵列分析鉴定出 358 个 RA 反应基因。mRNA 多样性分析显示，274 个基因通过选择性剪接产生多个蛋白编码转录本。在这 274 个基因中，我们选择了在 C 端具有 AS 的 26 个基因和 12 个转录因子基因进行进一步分析。通过使用转录物特异性引物，我们进行了定量实时 PCR 分析，以检查所有蛋白编码转录物的表达谱。结果，我们确定了基因，其蛋白编码转录物的表达在 RA 诱导下表现出不同的变化。
Zotero 插件