维甲酸诱导神经分化过程中过氧化物酶体蛋白转录本的表达增加。
The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation.
机构信息
Basic Medical Sciences Department, Islamic Azad University, Khorasgan Branch, Isfahan, Iran.
出版信息
Differentiation. 2011 Feb;81(2):127-32. doi: 10.1016/j.diff.2010.11.003. Epub 2010 Dec 9.
Peroxisomal matrix protein is mainly expressed in heart, skeletal muscle, and brain tissues. To study the expression of peroxisomal protein (PEP) during neurogenesis, we employed mouse embryonic carcinoma cells (P19) and embryonic stem cells (mESCs) as an in vitro model for neural differentiation by retinoic acid (RA) induction. Expression pattern of PEP was investigated under distinct steps of differentiation by RT-PCR and real-time PCR. The results revealed that expression of PEP transcripts was markedly increased after the RA treatment at embryoid body and neural stages. Therefore, we concluded that PEP might be involved in the early process of neurogenesis, which needs further verification.
过氧化物酶体基质蛋白主要表达于心、骨骼肌和脑组织中。为了研究过氧化物酶体蛋白（PEP）在神经发生过程中的表达情况，我们利用小鼠胚胎癌细胞（P19）和胚胎干细胞（mESCs）作为体外模型，通过视黄酸（RA）诱导进行神经分化。通过 RT-PCR 和实时 PCR 研究了 PEP 在不同分化阶段的表达模式。结果表明，在类胚体和神经阶段用 RA 处理后，PEP 转录本的表达明显增加。因此，我们得出结论，PEP 可能参与了神经发生的早期过程，这需要进一步验证。
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