Department of Psychiatry & Neuropsychology, School for Mental Health and Neurosciences, Maastricht University, NL-6200 MD Maastricht, The Netherlands.
Mech Ageing Dev. 2010 Sep;131(9):574-9. doi: 10.1016/j.mad.2010.08.002. Epub 2010 Aug 20.
Caloric restriction (CR) and antioxidants have been proposed as strategies to attenuate age-related brain changes. The hippocampus and its subregions dentate gyrus (DG), CA3 and CA1-2 show vulnerability to aging, with hippocampal volume alterations as a measurable sign. Using design-based stereological techniques, we investigated the volumes of the hippocampus and its subregions in six 12-month-old and six 24-month-old mice that were randomly selected from four aging cohorts of 60 male mice each: (1) wild-type mice (WT) fed with control diet (CD), (2) transgenic mice oxerexpressing normal human SOD1 fed with CD, (3) WT mice fed with CR diet, and (4) SOD1 mice fed with CR diet. Aging reduced the mean volume of the entire hippocampus (-9.5%), grey (-8.7%) and white matter (-9.7%), and CA3 subregion (-13.6%), but not DG or CA1-2 subregion. CR reduced the mean volumes of every hippocampal region investigated (on average -11%) in both 12-month-old, and 24-month-old mice. Overexpression of SOD1 was not associated with any volume alteration. These findings indicate that although aging and CR in mice are both associated with hippocampal volume reductions, the patterns of the volume reductions differ. These morphometric alterations may have impact on the function of the hippocampus during aging and CR.
热量限制（CR）和抗氧化剂被提议作为减轻与年龄相关的大脑变化的策略。海马体及其亚区齿状回（DG）、CA3 和 CA1-2 易受衰老影响，海马体体积变化是可测量的标志。使用基于设计的立体学技术，我们研究了从四个年龄组的 60 只雄性小鼠中随机选择的六只 12 个月大和六只 24 个月大的小鼠的海马体及其亚区的体积：（1） 野生型小鼠（WT）用对照饮食（CD）喂养，（2） 过表达正常人类 SOD1 的转基因小鼠用 CD 喂养，（3） WT 小鼠用 CR 饮食喂养，和（4） SOD1 小鼠用 CR 饮食喂养。衰老使整个海马体（-9.5%）、灰质（-8.7%）和白质（-9.7%）以及 CA3 亚区（-13.6%）的平均体积减少，但 DG 或 CA1-2 亚区没有减少。CR 减少了在 12 个月大和 24 个月大的小鼠中所有研究的海马区的平均体积（平均减少 11%）。SOD1 的过表达与任何体积变化无关。这些发现表明，尽管衰老和 CR 在小鼠中都与海马体体积减少有关，但体积减少的模式不同。这些形态测量学改变可能会对衰老和 CR 期间海马体的功能产生影响。
