Gao G L, Na W, Wang Y X, Zhang H F, Li H, Wang Q G
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China.
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China
Genet Mol Res. 2015 May 11;14(2):4847-57. doi: 10.4238/2015.May.11.17.
This study investigated the role of the chicken liver fatty acid-binding protein (L-FABP) gene in lipid metabolism in hepatocytes, and the regulatory relationships between L-FABP and genes related to lipid metabolism. The short hairpin RNA (shRNA) interference vector with L-FABP and an eukaryotic expression vector were used. Chicken hepatocytes were subjected to shRNA-mediated knockdown or L-FABP cDNA overexpression. Expression levels of lipid metabolism-related genes and biochemical parameters were detected 24, 36, 48, 60, and 72 h after transfection with the interference or overexpression plasmids for L-FABP, PPARα and L-BABP expression levels, and the total amount of cholesterol, were significantly affected by L-FABP expression. L-FABP may affect lipid metabolism by regulating PPARα and L-BABP in chicken hepatocytes.
本研究探讨了鸡肝脏脂肪酸结合蛋白（L-FABP）基因在肝细胞脂质代谢中的作用，以及L-FABP与脂质代谢相关基因之间的调控关系。使用了带有L-FABP的短发夹RNA（shRNA）干扰载体和真核表达载体。对鸡肝细胞进行shRNA介导的敲低或L-FABP cDNA过表达。在用L-FABP干扰或过表达质粒转染后24、36、48、60和72小时，检测脂质代谢相关基因的表达水平和生化参数，PPARα和L-BABP的表达水平以及胆固醇总量均受L-FABP表达的显著影响。L-FABP可能通过调节鸡肝细胞中的PPARα和L-BABP来影响脂质代谢。
