Nanobioelectronics & Biosensors Group, Institut Català de Nanotecnologia, CIN2 (CSIC), Campus UAB, Barcelona, Spain.
Biosens Bioelectron. 2010 Dec 15;26(4):1715-8. doi: 10.1016/j.bios.2010.07.090. Epub 2010 Jul 30.
A label-free bioelectronic detection of aptamer-thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5'amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5 mM Fe(CN)(6), obtaining a detection limit of 105 pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications.
本文报道了一种基于电化学阻抗谱（EIS）技术的无标记适体-凝血酶相互作用的生物电子检测方法。多壁碳纳米管（MWCNTs）被用作丝网印刷碳电极（SPCE）的修饰剂，与裸 SPCE 相比，显示出了更好的特性。5'氨基连接的适体序列被固定在修饰后的 SPCE 上，然后通过在存在 5mM [Fe(CN)（6）]（3-/4-）时对电荷转移电阻（Rct）的 EIS 转换来监测凝血酶与适体序列的结合，检测限为 105 pM。本研究代表了一种用于检测具有未来应用价值的蛋白质的替代电化学生物传感器。
