Department of Chemistry & Institute of Functional Materials, Inje University, 706 Obang-dong, Gimhae 621-749, South Korea.
Biosens Bioelectron. 2010 Dec 15;26(4):1576-81. doi: 10.1016/j.bios.2010.07.116. Epub 2010 Aug 5.
We have developed a monitoring system for evaluating the effect of anticancer agents, such as cisplatin and 5-fluorouracil (5-FU). The system mainly consisted of a quartz crystal microbalance (QCM) with indium tin oxide (ITO) electrodes and a micro CCD camera that can function in a humid CO(2) incubator. Human hepatoma cell line HepG2 cells were cultured and treated with the anticancer agents. As the behavior on the resonance frequency (F)-resonance resistance (R) diagram shows the viscoelastic change on the surface of the QCM, the effect of the anticancer agent was evaluated with the F-R diagram and the micro CCD camera, in comparison with the results in the case of general culturing (no anticancer agent injection). During general culturing, the resonance frequency decreased and the resonance resistance increased. This means that the mass loading of a viscous material occurred on the QCM. Observing with the micro CCD camera, the cancer cells were spread, divided, and the number of the cells increased. On the other hand, when the anticancer agent was injected to the culturing cancer cells, the resonance frequency and the resonance resistance increased continuously. This means a decrement of the mass effect and an increment of the viscosity on the QCM. From the observation with the micro CCD camera, the number of the cells did not change. The cells shrinked and changed the shape flat to round by loosing the cell activity in the case of 5-FU treatment. These results indicate the anticancer agents were effective to the culturing cancer cells.
我们开发了一种用于评估抗癌剂（如顺铂和 5-氟尿嘧啶（5-FU））效果的监测系统。该系统主要由带有铟锡氧化物（ITO）电极的石英晶体微天平（QCM）和可以在潮湿的 CO 2 孵育箱中工作的微型 CCD 相机组成。人肝癌细胞系 HepG2 细胞被培养并接受抗癌剂处理。由于共振频率（F）-共振电阻（R）图上的行为显示了 QCM 表面的粘弹性变化，因此可以通过 F-R 图和微型 CCD 相机评估抗癌剂的效果，与一般培养（未注射抗癌剂）的结果进行比较。在一般培养过程中，共振频率降低，共振电阻增加。这意味着 QCM 上发生了粘性物质的质量负载。用微型 CCD 相机观察，癌细胞扩散、分裂，细胞数量增加。另一方面，当抗癌剂注入培养的癌细胞时，共振频率和共振电阻不断增加。这意味着 QCM 上的质量效应减小，粘度增加。从微型 CCD 相机的观察结果来看，细胞数量没有变化。在 5-FU 处理的情况下，细胞通过降低细胞活性而收缩并将形状从扁平变为圆形。这些结果表明抗癌剂对培养的癌细胞有效。
