通过对M13 DNA进行特异性引物标记提高人类DNA指纹图谱的分辨率和灵敏度。

Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA.

作者信息

Tassanakajon A, Wongteerasapaya C, Pumichoti P, Boonsaeng V, Panyim S

机构信息

Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.

出版信息

Mol Cell Probes. 1991 Apr;5(2):111-5. doi: 10.1016/0890-8508(91)90004-4.

DOI:10.1016/0890-8508(91)90004-4

PMID:2072932

Abstract

A method to label M13 DNA probe by primer extension using a specific oligonucleotide primer is described. The method specifically labels the two 15-bp repeats in M13 DNA which hybridize to target DNA giving rise to DNA fingerprinting patterns. The M13 probe labelled by this method gave superior DNA fingerprinting patterns that that labelled by random primers. As little as 0.25 microgram of target DNA was sufficient for DNA fingerprinting. Non-isotopic labelling by the specific primer also showed improved DNA fingerprinting pattern. The results demonstrate the methodology to improve DNA fingerprinting based on M13 DNA probe.

摘要

描述了一种使用特定寡核苷酸引物通过引物延伸标记M13 DNA探针的方法。该方法特异性地标记M13 DNA中的两个15碱基对重复序列，这些重复序列与靶DNA杂交，产生DNA指纹图谱。用这种方法标记的M13探针产生的DNA指纹图谱比用随机引物标记的更好。低至0.25微克的靶DNA就足以进行DNA指纹分析。通过特异性引物进行的非同位素标记也显示出改进的DNA指纹图谱。结果证明了基于M13 DNA探针改进DNA指纹分析的方法。