M13生物印记：利用生物素化的M13噬菌体对个体特异性人类DNA指纹进行非同位素检测。

M13 Bioprints: non-isotopic detection of individual-specific human DNA fingerprints with biotinylated M13 bacteriophage.

作者信息

Medeiros A C, Macedo A M, Pena S D

机构信息

Department of Biochemistry and Immunology, Federal University of Minas Gerais, Brazil.

出版信息

Forensic Sci Int. 1989 Dec;43(3):275-80. doi: 10.1016/0379-0738(89)90155-2.

DOI:10.1016/0379-0738(89)90155-2

PMID:2613141

Abstract

M13 bacteriophage was labelled by primer extension with biotin-11-dUTP and was successfully used as probe to study human hypervariable minisatellites (DNA fingerprints) in Southern blots. The method, which we called M13 Bioprints, presents two advantages over conventional DNA fingerprinting. First, it is easily accessible, since it utilizes the widely available M13 phage as a probe. Second, it makes use of biotin, which is safer, simpler and more economical than isotopic labels.

摘要

M13噬菌体通过用生物素-11-dUTP进行引物延伸来标记，并成功用作探针，在Southern杂交中研究人类高变微卫星（DNA指纹）。我们将该方法称为M13生物印记法，它相对于传统的DNA指纹分析有两个优点。第一，它很容易获得，因为它利用了广泛可得的M13噬菌体作为探针。第二，它使用生物素，与同位素标记相比，生物素更安全、更简便且更经济。