School of Pharmacy, Faculty of Mathematics and Natural Sciences, Institute of Basic Medical Sciences, University of Oslo, N-0317 Oslo, Norway.
Glycobiology. 2010 Dec;20(12):1654-64. doi: 10.1093/glycob/cwq120. Epub 2010 Aug 20.
The leaves of the tree Opilia celtidifolia have a long tradition for being used in Mali and other West African countries against various ailments such as for wound healing. Previous studies on polysaccharides from these leaves showed the presence of pectic-like polymers with an effect on the human complement system as well as the ability to activate macrophages. The present study shows that bioactive arabinogalactans isolated by water of 50°C could be separated into two acidic fractions, Oc50A1 and Oc50A2. The former could, by gel filtration on Sephacryl S-400, be separated into two fractions, which were further purified on a Superdex 200 column to give the fractions Oc50A1.I.pur and Oc50A1.II.pur. These fractions were subjected to chemical and biological studies. The polysaccharides consisted mainly of heavily branched type II arabinogalactans and minor amounts of rhamnogalacturonan I regions. The isolated polymers had a high human complement-fixating ability, as well as the ability to stimulate rat macrophages and dendritic cells (DCs) and to induce B cell proliferation. These effects were especially pronounced for the higher molecular weight fraction of Oc50A1.I.pur. The fractions Oc50A1.I.pur and Oc501.II.pur stimulated secretion of pro-inflammatory cytokines from purified B cells or DCs. Collectively, these results indicate that the arabinogalactan type II polymers present in the leaves of O. celtidifolia may be used to develop medical devices for regulating inflammatory processes.
树 Opilia celtidifolia 的叶子在马里和其他西非国家有着悠久的传统，被用于治疗各种疾病，如伤口愈合。先前对这些叶子的多糖的研究表明，存在类似于果胶的聚合物，对人体补体系统有影响，并且能够激活巨噬细胞。本研究表明，通过 50°C 水分离的生物活性阿拉伯半乳聚糖可以分为两个酸性部分，Oc50A1 和 Oc50A2。前者可以通过 Sephacryl S-400 凝胶过滤进一步分离成两个部分，然后在 Superdex 200 柱上进一步纯化，得到 Oc50A1.I.pur 和 Oc50A1.II.pur 部分。这些部分进行了化学和生物学研究。多糖主要由高度支化的 II 型阿拉伯半乳聚糖和少量鼠李半乳糖醛酸聚糖 I 区组成。分离的聚合物具有高的人补体固定能力，以及刺激大鼠巨噬细胞和树突状细胞 (DC) 并诱导 B 细胞增殖的能力。对于 Oc50A1.I.pur 的高分子量部分，这些作用尤其明显。Oc50A1.I.pur 和 Oc501.II.pur 部分刺激从纯化的 B 细胞或 DC 分泌促炎细胞因子。总之，这些结果表明，存在于 O. celtidifolia 叶子中的 II 型阿拉伯半乳聚糖聚合物可用于开发用于调节炎症过程的医疗设备。
