Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P. O. Box 1068 Blindern, 0316 Oslo, Norway.
Department of Traditional Medicine, BP 1746, Bamako, Mali.
Molecules. 2014 Jun 6;19(6):7440-58. doi: 10.3390/molecules19067440.
The root bark, stem bark and leaves of Terminalia macroptera were sequentially extracted with ethanol, 50% ethanol-water, and 50 °C and 100 °C water using an accelerated solvent extractor. Ten bioactive purified polysaccharide fractions were obtained from those crude extracts after anion exchange chromatography and gel filtration. The polysaccharides and their native extracts were characterized with respect to molecular weight, chemical compositions and effects in the complement assay. The chemical compositions showed that the polysaccharides are of pectic nature. The results indicated that there was no great difference of the complement fixation activities in the crude extracts from the different plant parts when extracting with the accelerated solvent extraction system. The purified polysaccharide fractions 100WTSBH-I-I and 100WTRBH-I-I isolated from the 100 °C water extracts of stem and root bark respectively, showed the highest complement fixation activities. These two fractions have rhamnogalacturonan type I backbone, but only 100WTSBH-I-I contains side chains of both arabinogalactan type I and II. Based on the yield and activities of the fractions studied those from the root bark gave highest results, followed by those from leaves and stem bark. But in total, all plant materials are good sources for fractions containing bioactive polysaccharides.
采用加速溶剂萃取法，依次用乙醇、50%乙醇-水和 50°C、100°C 水从诃子的根皮、茎皮和叶中提取。经过阴离子交换色谱和凝胶过滤，从这些粗提取物中得到了 10 种具有生物活性的纯化多糖级分。对多糖及其天然提取物的分子量、化学成分和补体测定的影响进行了表征。化学成分表明多糖具有果胶性质。结果表明，采用加速溶剂萃取系统提取时，不同植物部位的粗提取物的补体固定活性没有很大差异。分别从茎皮和根皮的 100°C 水提取物中分离得到的纯化多糖级分 100WTSBH-I-I 和 100WTRBH-I-I，具有最高的补体固定活性。这两个级分具有鼠李半乳糖醛酸聚糖 I 型主链，但只有 100WTSBH-I-I 含有阿拉伯半乳聚糖 I 型和 II 型的侧链。基于研究的级分的产率和活性，根皮的产率最高，其次是叶和茎皮。但总的来说，所有植物材料都是含有生物活性多糖的级分的良好来源。
