费氏弧菌 LuxR N 端结构域是 GroEL 伴侣蛋白的作用靶点。
The N-terminal domain of Aliivibrio fischeri LuxR is a target of the GroEL chaperonin.
机构信息
State Research Institute of Genetics and Selection of Industrial Microorganisms Genetika, 1st Dorozhnii pr 1, 117545 Moscow, Russia.
出版信息
J Bacteriol. 2010 Oct;192(20):5549-51. doi: 10.1128/JB.00754-10. Epub 2010 Aug 20.
Abstract
Here we show that the C-terminal domain of LuxR activates the transcription of Aliivibrio fischeri luxICDABEG in Escherichia coli SKB178 gro(+) and E. coli OFB1111 groEL673 strains to the same level. Using affinity chromatography, we showed that GroEL binds to the N-terminal domain of LuxR, pointing to a GroEL/GroES requirement for the folding of the N-terminal domain of LuxR.
摘要
在这里，我们展示了 LuxR 的 C 末端结构域能够在大肠杆菌 SKB178 gro(+)和大肠杆菌 OFB1111 groEL673 菌株中激活 Aliivibrio fischeri luxICDABEG 的转录，其转录水平与在 Aliivibrio fischeri 中相同。通过亲和层析实验，我们发现 GroEL 能够与 LuxR 的 N 末端结构域结合，这表明 GroEL/GroES 对于 LuxR 的 N 末端结构域的折叠是必需的。
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